Zwitterionic polymers-armored amyloid-like protein surface combats thrombosis and biofouling  

作　　者：Xiaohui Mou Wan Miao Wentai Zhang Wenxuan Wang Qing Ma Zeyu Du Xin Li Nan Huang Zhilu Yang 

机构地区：[1]School of Materials Science and Engineering,Key Lab of Advanced Technology of Materials of Education Ministry,Southwest Jiaotong University,Chengdu,610031,China [2]Dongguan Key Laboratory of Smart Biomaterials and Regenerative Medicine,The Tenth Affiliated Hospital of Southern Medical University,Dongguan,Guangdong,523000,China [3]Department of Cardiology,Third People’s Hospital of Chengdu Affiliated to Southwest Jiaotong University,Chengdu,Sichuan 610072,China

出　　处：《Bioactive Materials》2024年第2期37-51,共15页生物活性材料（英文）

基　　金：supported by by the National Natural Science Foundation of China(Project 82202325,82072072,32171326,32261160372);the Guang Dong Basic and Applied Basic Research Foundation(2022B1515130010,2021A1515111035);China Postdoctoral Science Foundation(2022M721524).

摘　　要：Proteins,cells and bacteria adhering to the surface of medical devices can lead to thrombosis and infection,resulting in significant clinical mortality.Here,we report a zwitterionic polymers-armored amyloid-like protein surface engineering strategy we called as“armored-tank”strategy for dual functionalization of medical devices.The“armored-tank”strategy is realized by decoration of partially conformational transformed LZM(PCTL)assembly through oxidant-mediated process,followed by armoring with super-hydrophilic poly-2-methacryloyloxyethyl phosphorylcholine(pMPC).The outer armor of the“armored-tank”shows potent and durable zone defense against fibrinogen,platelet and bacteria adhesion,leading to long-term antithrombogenic properties over 14 days in vivo without anticoagulation.Additionally,the“fired”PCTL from“armored-tank”actively and effectively kills both Gram-positive and Gram-negative bacterial over 30 days as a supplement to the lacking bactericidal functions of passive outer armor.Overall,this“armored-tank”surface engineering strategy serves as a promising solution for preventing biofouling and thrombotic occlusion of medical devices.

关 键 词：Amyloid-like protein ANTIFOULING ANTITHROMBOSIS SUPER-HYDROPHILICITY Zwitterionic polymers 

分 类 号：R318[医药卫生—生物医学工程]