总蛋白苏木化修饰与男性甲状腺乳头状癌的初步探究  

Correlationship between total proteins SUMOylation and papillary thyroid carcinoma in males

作　　者：吴巧 刘威 郑佼皎 王聪[1] 艾志龙[1] WU Qiao;LIU Wei;ZHENG Jiaojiao;WANG Cong;AI Zhilong(Department of Thyroid-Breast Surgery,Zhongshan Hospital,Fudan University,Shanghai 200032,China)

机构地区：[1]复旦大学附属中山医院甲乳外科,上海200032

出　　处：《外科理论与实践》2024年第4期336-344,共9页Journal of Surgery Concepts & Practice

基　　金：上海市“科技创新行动计划”(21ZR1412000)。

摘　　要：目的:探究蛋白质苏木化修饰水平(SUMOylation)与男性甲状腺乳头状癌(papillary thyroid carcinoma,PTC)预后的关系。方法:首先通过数据库与生物信息学分析,后续收集本地男性PTC病人手术切除的癌和癌旁组织配对样本,免疫组织化学(immunohistochemistry,IHC)、蛋白质印迹(Western blotting,WB)检测样本中蛋白质的苏木化水平,利用实时荧光定量聚合酶链反应(quantitative real⁃time polymerase chain reaction,qRT⁃PCR)检测样本中苏木化修饰相关因子的mRNA表达水平,比较癌组织与癌旁组织中蛋白质苏木化水平以及苏木化通路关键因子的mRNA表达水平,研究苏木化修饰与男性PTC的关系。结果:TCGA与GTEx数据库分析发现蛋白质苏木化水平在PTC的癌组织中呈现升高趋势(P<0.05),并与病人不良预后相关(P=0.021)。本院临床样本中验证了癌组织蛋白苏木化修饰水平高于对应的癌旁组织(P<0.05);但是qRT⁃PCR显示苏木化通路中大部分因子的转录水平无明显变化。结论:PTC组织中蛋白质苏木化水平高于癌旁组织,癌组织中蛋白质苏木化水平越高,则病人生存时间越短。Objective To investigate the relationship between protein SUMOylation level and the prognosis of papillary thyroid carcinoma(PTC)in males.Methods Protein SUMOylation levels in PTC was analyzed by bioinformatics based on GTEx and TCGA databases and validated by immunohistochemical staining and Western blotting in our clinical pairs specimens.The mRNA expression of the protein SUMOylation associated genes were measured by fluorescent quantitative real⁃time polymerase chain reaction(qRT⁃PCR)in surgical pairs specimens.Results The expression level of SUMOylation in the tumor tissues of PTC showed an elevated trend(P<0.05),and was associated with poor prognosis of the patients by TCGA and GTEx databases analysis(P=0.021).In the clinical samples of our hospital,it was verified that the level of SUMOylation in tumor tissues was higher than that of the paired non⁃tumor tissues(P<0.05).However,qRT⁃PCR showed no significant changes in the transcriptional level of the protein SUMOylation associated genes in most cases.Conclusions Protein SUMOylation in thyroid tumor tissues were higher than that in paired non⁃tumor tissues,and the higher SUMOylation levels levels in tumor tissues were,the shorter overall survival time of the patients was.

关 键 词：苏木化修饰 男性 甲状腺乳头状癌 

分 类 号：R736.1[医药卫生—肿瘤]