An improved method for tubulin staining of ciliated eukaryotes  

作　　者：Tingting Pan Jing Zhang Che Hu Guanxiong Yan Weiwei Qin Zixin Cheng Dongxia Yuan Fang Zhou Jie Xiong Wei Miao Chuanqi Jiang 

机构地区：[1]Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan,China [2]University of Chinese Academy of Sciences,Beijing,China [3]Harbin Normal University,Harbin,China [4]Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture,Chinese Academy of Sciences,Wuhan,China [5]Hubei Hongshan Laboratory,Wuhan,China [6]Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation,Beibu Gulf University,Qinzhou,China

出　　处：《Water Biology and Security》2024年第3期65-71,共7页水生生物与安全（英文）

基　　金：supported by the Third Xinjiang Scientific Expedition Program(2021xjkk0204,2021xjkk0604);Science&Technology Fundamental Resources Investigation Program(2022FY100400);Background Resources Survey in Shennongjia National Park(SNJNP2022008);Hubei Provincial Key Laboratory for Conservation Biology of Shennongjia Snub-nosed Monkeys(SNJGKL2022008);Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation,Beibu Gulf University(2020KA05).

摘　　要：Ciliated microeukaryotes(ciliates)are distinguished by their cilia,which are rich in tubulin.We developed a method for tubulin staining in ciliate cells that involves using live-cell tubulin-staining dyes instead of antibodies thereby streamlining the staining process,which is effective across diverse ciliate lineages.Moreover,our method allows integration with immunofluorescence staining using antibodies when needed.The potential applications of this technique extend to cell biology and ciliate morphological and ecological studies.

关 键 词：CILIATE CILIUM Live-cell tubulin dye Tubulin staining 

分 类 号：Q95[生物学—动物学]