Repeated platelet-rich plasma injections improve erectile dysfunction in a rat model of hyperhomocysteinemia  

作　　者：Zhe Yu Yuan-Zhi Xie Xiao-Lan Huang Su-Zhen Huang Xiang-Min Luo Jin-Xiang Wu 

机构地区：[1]Reproductive Medicine Center,The Second Affiliated Hospital of Fujian Medical University,Quanzhou 362000,China

出　　处：《Asian Journal of Andrology》2024年第6期622-627,共6页亚洲男性学杂志（英文版）

基　　金：supported by grants from the Quanzhou Science and Technology Project Funding(No.2021N017S);the Doctoral Nursery Fund of the Second Affiliated Hospital of Fujian Medical University(BS202102).

摘　　要：Platelet-rich plasma (PRP) shows promise as a regenerative modality for mild-to-moderate erectile dysfunction (ED). However, its efficacy in treating severe ED remains unknown. Blood samples from 8-week-old male rats were used to prepare PRP through a two-step centrifugation procedure, followed by chitosan activation and freeze thaw cycle. A hyperhomocysteinemia (HHcy)-related ED model was established using a methionine-enriched diet, and an apomorphine (APO) test was conducted during the 4th week. APO-negative rats were divided into two groups and were injected with PRP or saline every 2 weeks. Erectile function and histological analyses of the corpus cavernosum were performed during the 16th week. The results revealed that erectile function was significantly impaired in rats with HHcy-related ED compared to that in age-matched rats but was improved by repeated PRP injections. Immunofluorescence staining revealed a reduction in reactive oxygen species and additional benefits on the recovery of structures within the corpus cavernosum in rats that received PRP treatment compared to those in the saline-injected control group. Therefore, PRP could enhance functional and structural recovery in a severe HHcy-related ED model. A notable strength of the present study lies in the use of a repeated intracavernous injection method, mirroring protocols used in human studies, which offers more reliable results for translating the findings to humans.

关 键 词：erectile dysfunction growth factor HYPERHOMOCYSTEINEMIA platelet-rich plasma 

分 类 号：R697[医药卫生—泌尿科学]