Seminal plasma S100A8/A9 as a potential biomarker of genital tract inflammation  

作　　者：Qiu-Zi Shen Yong-Feng Wang Yi-Wei Fang Yuan-Yao Chen Li-Ting He Yuan Zhang Guo-Tao Liu Kai Zhao Chun-Yan Liu Zun-Pan Fan Hui-Ping Zhang 

机构地区：[1]Institute of Reproductive Health,Center for Reproductive Medicine,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China [2]The Affiliated Jinyang Hospital of Guizhou Medical University,Guiyang 550018,China [3]NHC Key Laboratory of Birth Defects Prevention,Henan Institute of Reproduction Health Science and Technology,Zhengzhou 450002,China [4]Center for Reproductive Medicine,The first Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China

出　　处：《Asian Journal of Andrology》2024年第5期464-471,共8页亚洲男性学杂志（英文版）

基　　金：supported by the National Natural Foundation of China(81871148);the Open Fund of NHC Key Laboratory of Birth Defects Prevention,Henan Key Laboratory of Population Defects Prevention(Henan Institute of Reproduction Health Science and Technology,ZD202201);the Fundamental Research Funds for the Central Universities,HUST(2023JYCXJJ062 and YCJJ202201050).

摘　　要：Infections and inflammatory reactions in the male genital tract are the leading causes of male infertility with a prevalence of 6%-10%,primarily affecting testicular and epididymal function and ultimately compromising sperm quality.However,most infertile patients with genital infection/inflammation are asymptomatic and easily overlooked.Traditional indicators,including white blood cells,elastase,and other components in semen,can reflect inflammation of the genital tract,but there is still a lack of a uniform standard method of detection.Therefore,it is necessary to explore reliable markers in semen that reflect the inflammatory status of the genital tract.Using the experimental autoimmune orchitis(EAO)model to simulate noninfectious chronic orchitis,we successfully collected ejaculated seminal fluid from EAO rats using optimized electrical stimulation devices.Proteomic analysis was performed using isobaric tags for relative and absolute quantification(iTRAQ).Compared to the control group,55 upregulated and 105 downregulated proteins were identified in seminal plasma samples from the EAO group.In a preliminary screening,the inflammation-related protein S100A8/A9 was upregulated.We further verified that S100A8/A9 was increased in seminal plasma and highly expressed in testicular macrophages of the EAO model.In patients with oligoasthenospermia and genital tract infections,we also found that S100A8/A9 levels were remarkably increased in seminal plasma and testicular macrophages.S100A8/A9 in semen may be a potential biomarker for chronic genital inflammation.Our study provides a new potential biomarker for early diagnosis and further understanding of male infertility caused by genital inflammation.

关 键 词：experimental autoimmune orchitis genital inflammation male infertility PROTEOMICS S100A8/A9 

分 类 号：R697[医药卫生—泌尿科学]