Safety of low-intensity extracorporeal shock wave therapy in prostate disorders:in vitro and in vivo evidence  

作　　者：Yi-Ran Wang Bin Feng Wen-Bo Qi Yu-Wen Gong Xiang-Bin Kong Hui Cheng Zhi-Long Dong Jun-Qiang Tian Zhi-Ping Wang 

机构地区：[1]Institute of Urology,Gansu Province Clinical Research Center for Urinary System Disease,The Second Hospital and Clinical Medical School,Lanzhou University,Lanzhou 730000,China [2]Department of Urology,Gansu Provincial Hospital,Lanzhou 730000,China [3]Department of Radiotherapy,First Affiliated Hospital of Zhengzhou University,Zhengzhou 450000,China [4]Department of Urology,Gansu Provincial Second People’s Hospital,Lanzhou 730000,China

出　　处：《Asian Journal of Andrology》2024年第5期535-543,共9页亚洲男性学杂志（英文版）

基　　金：supported by grants from the National Natural Science Foundation of China(No.PR0121015);the National Natural Science Foundation of China(No.82160148);the Natural Science Foundation Project of Gansu Province(No.2106RJZA110).

摘　　要：Recent evidence suggests that low-intensity extracorporeal shock wave therapy(Li-ESWT)is a promising treatment for chronic prostatitis/chronic pelvic pain syndrome(CP/CPPS);however,its safety in pelvic organs,particularly prostate tissues and cells,remains unclear.The current study evaluates the risks of prostate cell damage or oncogenesis following the administration of LiESWT for prostatitis.To this end,a robust in vitro model(Cell Counting Kit-8[CCK-8]assay,clone formation assay,cell scratch assay,lactate dehydrogenase[LDH]release assay,flow cytometry,and immunoblotting assay)was designed to examine the effects of Li-ESWT on cell proliferation,clonogenicity,migration,membrane integrity,and DNA damage.Exome sequencing of Li-ESWTtreated cells was performed to determine the risk of carcinogenesis.Furthermore,an in vivo rat model(n=20)was employed to assess the effects of Li-ESWT on cancer biomarkers(carcinoembryonic antigen[CEA],Ki67,proliferating cell nuclear antigen[PCNA],and gamma-H2A histone family member X,phosphorylation of the H2AX Ser-139[γ-H2AX])in prostate tissue.Based on our findings,Li-ESWT promotes cellular growth and motility without inducing significant cell membrane or DNA damage or alterations.Genetic analyses did not demonstrate an increase in mutations,and no damage to prostate tissue or upregulation of cancer biomarkers was detected in vivo.This comprehensive in vitro and in vivo assessment confirms the safety of Li-ESWT in managing prostate disorders.

关 键 词：CARCINOGENESIS chronic pelvic pain syndrome extracorporeal shock wave therapy PROSTATITIS SAFETY 

分 类 号：R687.3[医药卫生—骨科学]