Effects of acupuncture on iron metabolism in the hippocampus of the rats with cerebral ischemia-reperfusion injury based on the imbalance of iron homeostasis  

基于铁稳态的失衡探讨针刺对脑缺血再灌注损伤大鼠海马区铁代谢的影响

作　　者：Hong-Juan WANG Hong TANG Shan-Shan JIANG Zhan-Fu LI Hao-mei TIAN Chu-Tao CHEN 汪红娟;唐红;江姗姗;李展富;田浩梅;陈楚淘(湖南中医药大学针灸推拿与康复学院,长沙410208)

机构地区：[1]School of Acupuncture-moxibustion,Tuina and Rehabilitation,Hunan University of Chinese Medicine,Changsha 410208,China

出　　处：《World Journal of Acupuncture-Moxibustion》2024年第4期292-299,共8页世界针灸杂志（英文版）

基　　金：Supported by the Project of National Natural Science Fund of China:81874508,82274662;the Project of Natural Science Fund of Hunan Province:2021J304905;the Project of Natural Science Fund of Changsha Science and Technology Ministry of Hunan Province:kq2014094.

摘　　要：Objective:This study aimed to observe the effects of acupuncture on iron metabolism in the hippocampus of rats with cerebral ischemia-reperfusion injury(CIRI)and to explore the mechanism of acupuncture on neural repairinCIRIrats.Methods:After general feeding for 3 days,16 of 48 healthy Sprague-Dawley rats were selected according to a random number table to form a sham-occlusion group,and the models were prepared in the remaining rats.After successful modeling,the rats were randomized into model and acupuncture groups,with 16 rats in each group.The CIRI models were prepared using the modified Zea Longa suture-occlusion method.In the acupuncture group,the rats were fixed and acupuncture was delivered at“Shuigou"(GV26),“Dazhui"(GV14),and“Baihui"(GV20),and the needles were manipulated once every 15 min.In the sham-occlusion and model groups,rats were fixed without acupuncture.The intervention was performed for 30 min in each group,once every 12 h,seven times.The degree of neurological impairment was assessed by using the modified Garcia score.Cerebral blood flow(CBF)was monitored using a laser speckle blood flow imaging system.The cerebral infarction area ratio was measured using triphenyltetrazolium chloride staining.Ferric ion aggregation in brain tissue was noted using Prussian blue staining.The ferric ion and glutathione(GSH)contents of the hippocampal tissue on the ischemic side were detected using ferric ion and GSH kits.The protein expression of transferrin receptor 1(TFR1),iron regulatory protein 2(IRP2),and ferroportin(FPN)was detected via western blotting.Results:(1)Before intervention,compared with the sham-occlusion group,the Garcia neurological score was significantly reduced in the model and acupuncture groups(both P<0.01).After the intervention,compared with the sham-occlusion group,the Garcia neurological score in the model group decreased significantly(P<0.01),and the score in the acupuncture group was elevated compared with the model group(P<0.o5).Compared to before the intervention,the Garcia

关 键 词：ACUPUNCTURE Iron homeostasis Cerebral ischemia-reperfusion injury Ferroptosis TRANSFERRIN RATS 

分 类 号：R245[医药卫生—针灸推拿学]