梓醇对类风湿性关节炎大鼠软骨损伤的影响  

Effect of Catalpol on Cartilage Damage in Rheumatoid Arthritis Rats

作　　者：尹伟 杨立峰 于洋 宋永财 黄军刚 YIN Wei;YANG Lifeng;YU Yang;SONG Yongcai;HUANG Jungang(Hospital,Hanzhong 723000,Shaanxi China)

机构地区：[1]三二〇一医院,陕西汉中723000

出　　处：《中国中医骨伤科杂志》2024年第11期5-10,共6页Chinese Journal of Traditional Medical Traumatology & Orthopedics

摘　　要：目的:探究梓醇(Cat)对类风湿性关节炎(RA)大鼠软骨损伤的影响。方法:注射不完全弗氏佐剂鸡Ⅱ型胶原蛋白以建立类风湿性关节炎大鼠模型,将大鼠随机分为假手术组、模型组、梓醇低剂量(Cat-L)组、梓醇高剂量(Cat-H)组、Cat-H+Dorsomorphin(AMPK抑制剂)组。评估关节炎指数;ELISA法检测血清IL-1β、IL-6、TNF-α、CTX-Ⅰ、CTX-Ⅱ水平;HE染色观察软骨组织形态;TUNEL检测大鼠软骨肿胀细胞凋亡;检测软骨组织丙二醛(MDA)含量、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性;Western Blot检测关节软骨组织Cleaved-caspase-3、MMP-3、MMP-13、AMPK、NLRP3蛋白表达。结果:治疗结束后,与假手术组相比,模型组大鼠软骨组织明显被破坏,伴有软骨脱落;关节炎指数评分,血清IL-1β、IL-6、TNF-α、CTX-Ⅰ、CTX-Ⅱ水平,软骨组织细胞凋亡率,丙二醛含量,Cleaved-caspase-3、MMP-3、MMP-13、NLRP3蛋白表达水平显著升高,软骨组织过氧化氢酶和超氧化物歧化酶活性、AMPK蛋白表达水平显著降低,差异有统计学意义(P<0.05)。与模型组相比,梓醇低剂量组和梓醇高剂量组大鼠软骨组织形态有明显改善,关节炎指数评分、血清IL-1β、IL-6、TNF-α、CTX-Ⅰ、CTX-Ⅱ水平,软骨组织细胞凋亡率,丙二醛含量、Cleaved-caspase-3、MMP-3、MMP-13、NLRP3蛋白表达水平显著降低,软骨组织过氧化氢酶和超氧化物歧化酶活性、AMPK蛋白表达水平显著升高,差异有统计学意义(P<0.05)。Dorsomorphin可减弱梓醇对类风湿性关节炎大鼠软骨的保护作用,差异有统计学意义(P<0.05)。结论:梓醇可能通过调节AMPK/NLRP3信号通路降低类风湿性关节炎大鼠炎症、氧化应激反应和软骨细胞凋亡,减轻软骨损伤。Objective:To investigate the effect of catalpol(Cat)on cartilage damage in rats with rheumatoid arthritis(RA).Methods:Incomplete Freund's adjuvant chicken typeⅡcollagen was injected to rats to establish RA model.The rats were randomly separated into sham group,model group,Cat-L low-dose group,Cat-H high-dose group,and Cat-H+Dorsomorphin(AMPK inhibitor)group.The arthritis index was evaluated.ELISA method was applied to detect serum levels of IL-1β,IL-6,TNF-α,CTX-Ⅰ,and CTX-Ⅱ.HE staining was applied to observe the morphology of cartilage tissue.TUNEL was applied to detect apoptosis of rat cartilage swelling cells.The MDA content,SOD and CAT activities of cartilage tissue were detected.Western Blot was applied to detect the expression of Cleaved-caspase-3,MMP-3,MMP-13,AMPK,and NLRP3 proteins in articular cartilage tissue.Results:After treatment,compared with the sham group,the cartilage tissue of rats in the model group was greatly damaged,accompanied by cartilage detachment;the arthritis index score,levels of serum IL-1β,IL-6,TNF-α,CTX-Ⅰ,CTX-Ⅱ,chondrocyte apoptosis rate,content of MDA,and expression levels of Cleaved-caspase-3,MMP-3,MMP-13,and NLRP3 proteins were greatly increased;the activities of CAT and SOD in cartilage tissue,and the expression level of AMPK protein were greatly reduced(P<0.05).Compared with the model group,there was a great improvement in the morphology of cartilage tissue of rats in the Cat-L and Cat-H groups;the arthritis index score,levels of serum IL-1β,IL-6,TNF-α,CTX-Ⅰ,CTX-Ⅱ,chondrocyte apoptosis rate,content of MDA,and expression levels of Cleaved-caspase-3,MMP-3,MMP-13,and NLRP3 proteins in the Cat-L and Cat-H groups were greatly reduced;the activities of CAT and SOD in cartilage tissue,and the expression level of AMPK protein were in the Cat-L and Cat-H groups greatly increased(P<0.05).Dorsomorphin can weaken the protective effect of Cat on the cartilage of RA rats(P<0.05).Conclusion:Cat may reduce inflammation,oxidative stress,and chondrocyte apoptosis in RA rats an

关 键 词：梓醇 类风湿性关节炎 软骨损伤 凋亡 

分 类 号：R-33[医药卫生]