Evaluating the performance of the Alere PBP2a SA Culture Colony Test with the Vitek 2 Antimicrobial Susceptibility Test Card System as reference standard in coagulase-negative Staphylococcus species  

作　　者：Tze Shien Lo Michihiko Goto Kimberly D.P.Hammer 

机构地区：[1]Fargo VA Health Care System,2101 Elm Street,Fargo,ND 58102,USA [2]University of North Dakota School of Medicine&Health Sciences,1919 Elm Street,Fargo,ND 58102,USA [3]Iowa City VA Health Care System,601 Highway 6 West,Iowa City,IA 52246,USA [4]University of Iowa Carver College of Medicine,451 Newton Road,Iowa City,IA 52242,USA

出　　处：《Infectious Medicine》2024年第3期69-72,共4页感染医学（英文）

摘　　要：Background The Alere PBP2a SA Culture Colony Test is an FDA-cleared in vitro immunochromatographic assay for rapid detection of penicillin-binding protein2a(PBP2a)in Staphylococcus aureus.Methods We investigated the performance of the PBP2a SA Culture Colony Test with 78 coagulase-negative Staphylococcus(CoNS)isolates from different body sites,with the Vitek 2 Antimicrobial Susceptibility Test(AST)as a reference standard.Results The CoNS species were 62 S.epidermidis;6 S.lugdenensis;3 S.hominis;2 S.capitis;2 S.haemolyticus;and 1 each of S.simulans,S.auricularis,and S.warneri.Of the 78 CoNS isolates,68 showed concordance in the PBP2a IC assay and Vitek 2 AST.Discordance was seen for 10 S.epidermidis isolates,which showed negative in the PBP2a assay,despite oxacillin-resistance detection using the Vitek 2 AST(66.7%sensitivity and 100%specificity).All non-S.epidermidis CoNS were identified with 100%concordance using the PBP2a IC assay and Vitek 2 AST.Conclusion We demonstrated that,while the PBP2a IC assay has low sensitivity in determining the susceptibility of S.epidermidis to oxacillin,it highly accurately predicted the susceptibility of non-S.epidermidis CoNS to oxacillin.The diagnostic accuracy for non-S.epidermidis CoNS needs further assessment with more isolates to confirm our findings.

关 键 词：PBP2a IC assay Staphylococcus epidermidis Non-epidermidis coagulase-negative STAPHYLOCOCCUS 

分 类 号：R51[医药卫生—内科学]