Unravelling the physicochemical and antimicrobial mechanisms of human serum albumin/tannic acid coatings for medical-grade polycaprolactone scaffolds  

作　　者：Silvia Cometta Bogdan C.Donose Alfredo Juarez-Saldivar Akhilandeshwari Ravichandran Yanan Xu Nathalie Bock Tim R.Dargaville Aleksandar D.Rakic Dietmar W.Hutmacher 

机构地区：[1]Faculty of Engineering,School of Mechanical,Medical and Process Engineering,Queensland University of Technology,Brisbane,QLD,4000,Australia [2]Australian Research Council Training Centre for Multiscale 3D Imaging,Modelling and Manufacturing(M3D Innovation),Queensland University of Technology,Kelvin Grove,QLD,4059,Australia [3]Max Planck Queensland Centre,Queensland University of Technology,Brisbane,QLD,4000,Australia [4]School of Electrical Engineering and Computer Science,The University of Queensland,Brisbane,QLD,4072,Australia [5]Unidad Academica Multidisciplinaria Reynosa Aztlan,Universidad Autonoma de Tamaulipas,Reynosa,88740,Mexico [6]Central Analytical Research Facility(CARF),Queensland University of Technology,Brisbane,QLD,4000,Australia [7]Faculty of Health,School of Biomedical Sciences,Queensland University of Technology,Brisbane,QLD,4000,Australia [8]Translational Research Institute,Woolloongabba,QLD,4102,Australia [9]Centre for Materials Science,School of Chemistry and Physics,Queensland University of Technology,Brisbane,QLD,4000,Australia [10]Australian Research Council Training Centre for Cell and Tissue Engineering Technologies,Queensland University of Technology,Brisbane,QLD,4059,Australia

出　　处：《Bioactive Materials》2024年第12期68-84,共17页生物活性材料（英文）

基　　金：supported by the ARC Industrial Transformation Training Centre for Multiscale 3D Imaging,Modelling,and Manufacturing[IC 180100008],NHMRC 2008018-Transformation of the implant paradigm in breast rehabilitation Grant;the Max Planck Queensland Centre.

摘　　要：Biofilm-related biomaterial infections are notoriously challenging to treat and can lead to chronic infection and persisting inflammation.To date,a large body of research can be reviewed for coatings which potentially prevent bacterial infection while promoting implant integration.Yet only a very small number has been translated from bench to bedside.This study provides an in-depth analysis of the stability,antibacterial mechanism,and biocompatibility of medical grade polycaprolactone(mPCL),coated with human serum albumin(HSA),the most abundant protein in blood plasma,and tannic acid(TA),a natural polyphenol with antibacterial properties.Molecular docking studies demonstrated that HSA and TA interact mainly through hydrogen-bonding,ionic and hydrophobic interactions,leading to smooth and regular assemblies.In vitro bacteria adhesion testing showed that coated scaffolds maintained their antimicrobial properties over 3 days by significantly reducing S.aureus colonization and biofilm formation.Notably,amplitude modulation-frequency modulation(AMFM)based viscoelasticity mapping and transmission electron microscopy(TEM)data suggested that HSA/TA-coatings cause morphological and mechanical changes on the outer cell membrane of s.aureus leading to membrane disruption and cell death while proving non-toxic to human primary cells.These results support this antibiotic-free approach as an effective and biocompatible strategy to prevent biofilm-related biomaterial infections.

关 键 词：Bacterial infection BIOFILM Human serum albumin Tannic acid POLYCAPROLACTONE 3D printing SCAFFOLD 

分 类 号：R318[医药卫生—生物医学工程]