Versatile effects of galectin-1 protein-containing lipid bilayer coating for cardiovascular applications  

作　　者：Md Lemon Hasan Ju Ro Lee Khandoker Asiqur Rahaman Dae Hyeok Yang Yoon Ki Joung 

机构地区：[1]Center for Biomaterials,Biomedical Research Institute,Korea Institute of Science and Technology(KIST),Hwarangno 14-gil 5,Seongbuk-gu,Seoul,02792,Republic of Korea [2]Division of Bio-Medical Science&Technology,University of Science and Technology(UST),113 Gwahangno,Yuseong-gu,Daejeon,34113,Republic of Korea [3]Center for Systems Biology,Massachusetts General Hospital Research Institute,Boston,MA,02114,USA [4]Department of Radiology,Massachusetts General Hospital,Harvard Medical School,Boston,MA,02114,USA [5]Institute of Cell and Tissue Engineering,College of Medicine,The Catholic University of Korea,Seoul,06591,Republic of Korea [6]KHU-KIST Department of Conversing Science and Technology,Graduate School,Kyung Hee University,Seoul,Republic of Korea

出　　处：《Bioactive Materials》2024年第12期207-225,共19页生物活性材料（英文）

基　　金：supported by grants the Nano Material Technology Development Program(NRF-2021M3H4A1A04092879)through the National Research Foundation of Korea(NRF)funded by the Ministry of Science and ICT;the Materials and Parts Technology Development Program(20023353);Advanced Technology Center(ATC+,20017939)funded by the Ministry of Trade,Industry&Energy(MOTIE,Korea).

摘　　要：Modulating inflammatory cells in an implantation site leads to severe complications and still unsolved challenges for blood-contacting medical devices.Inspired by the role of galectin-1(Gal-1)in selective functions on multiple cells and immunomodulatory processes,we prepared a biologically target-specific surface coated with the lipid bilayer containing Gal-1(Gal-1-SLB)and investigate the proof of the biological effects.First,lipoamido-dPEG-acid was deposited on a gold-coated substrate to form a self-assembled monolayer and then conjugated dioleoylphosphatidylethanolamine(DOPE)onto that to produce a lower leaflet of the supported lipid bilayer(SLB)before fusing membrane-derived vesicles extracted from B16-F10 cells.The Gal-1-SLB showed the expected anti-fouling activity by revealing the resistance to protein adsorption and bacterial adhesion.In vitro studies showed that the Gal-1-SLB can promote endothelial function and inhibit smooth muscle cell proliferation.Moreover,Gal-1-SLB presents potential function for endothelial cell migration and angiogenic activities.In vitro macrophage culture studies showed that the Gal-1-SLB attenuated the LPS-induced inflammation and the production of macrophage-secreted inflammatory cytokines.Finally,the implanted Gal-1-SLB reduced the infiltration of immune cells at the tissue-implant interface and increased markers for M2 polarization and blood vessel formation in vivo.This straightforward surface coating with Gal-1 can be a useful strategy for modulating the vascular and immune cells around a blood-contacting device.

关 键 词：Galectin-1 protein Supported lipid bilayer MACROPHAGE Cardiovascular devices Inflammation 

分 类 号：R54[医药卫生—心血管疾病]