Deciphering 3D periodontal fibroblast-macrophage crosstalk in bioactive nanoparticle-guided immunomodulation for treating traumatic dental avulsion  

作　　者：Hadagalu Revana Siddappa Rajeshwari Emily Bishop Aiman Ali Anil Kishen 

机构地区：[1]The Kishen Lab,Dental Research Institute,University of Toronto,Toronto,ON,M5G 1G6,Canada [2]Faculty of Dentistry,University of Toronto,Toronto,ON,M5G 1G6,Canada [3]Oral and Maxillofacial Pathology and Oral Medicine,Faculty of Dentistry,University of Toronto,124 Edward Street,Toronto,Ontario M5G 1G6,Canada [4]Department of Dentistry,Mount Sinai Health System,Mount Sinai Hospital,Toronto,ON,M5G 1X5,Canada

出　　处：《Bioactive Materials》2024年第11期400-412,共13页生物活性材料（英文）

基　　金：American Association of Endodontists Foundation(#1208771);Natural Sciences and Engineering Research Council of Canada(Discovery grant)(AK-RGPIN-2020-05844);Canada Research Chair Program,and Dr.Lloyd and Mrs.Kay Chapman Chairship.

摘　　要：Prolonged extra-oral period in dental avulsion is often associated with loss of viability of Periodontal fibroblasts (PDLF) and increased risk of ankylosis. Root surface treatment with bioactive agents to reduce the risk of ankylosis can be a potential strategy. The objective of the study was to investigate the impact of an engineered chitosan nanoparticles (CSNP), photosensitizer Rose Bengal (RB) functionalized CSNP (CSRB) and sustained dexamethasone (CSDEX) releasing CSNP for application in management of delayed replantation of avulsed teeth. The 3D PDLF-macrophage (Mϕ) collagen model was developed and exposed to LPS, MCSF, RANKL with and without CSDEX/CSNP. Immunofluorescence and cytokine analysis was done at 2 and 7 days to assess cellular interactions. Maxillary right incisors in male Wistar rats were extracted, exposed to extraoral dry or LPS for 1 h and treated with or without CSDEX/CSRB for 1 min before replantation. Rats were euthanized after 21 days for micro-CT, TRAP, and immunofluorescence analysis. CSDEX/CSNP treatment in 3D model significantly reduced CD80, NFATc1, STAT6 and increased CD206 and periostin expression (p < 0.05). TNFα, MMP9 was downregulated and IL10, TGFβ1, MMP2 upregulated with CSDEX/CSNP (p < 0.05). CSDEX/CSRB in animal study significantly reduced resorption, ankylosis, TRAP activity and osteocalcin expression and increased periostin (p<0.05). CSDEX demonstrated higher anti-inflammatory activity by downregulating TNFα, while CSNP upregulated TGFβ1, periostin, and downregulated MMP9. The combination of matrix stabilization with CSRB with periostin upregulation and sustained releasing CSDEX showed potential for hampering root resorption and ankylosis in dental avulsion.

关 键 词：Root resorption Chitosan nanoparticles IMMUNOMODULATION Periodontal fibroblasts Macrophages 

分 类 号：R318[医药卫生—生物医学工程]