Suspension electrospinning of decellularized extracellular matrix: A new method to preserve bioactivity  

作　　者：Sarah Jones Sabrina VandenHeuvel Andres Luengo Martinez Ruchi Birur Eric Burgeson Isabelle Gilbert Aaron Baker Matthew Wolf Shreya A.Raghavan Simon Rogers Elizabeth Cosgriff-Hernandez 

机构地区：[1]Department of Biomedical Engineering,The University of Texas at Austin,Austin,TX,78712,USA [2]Department of Biomedical Engineering,Texas A&M University,College Station,TX,77843,USA [3]Department of Chemical and Biomolecular Engineering,University of Illinois Urbana-Champaign,Champaign,IL,61820,USA [4]Cancer Biomaterials Engineering Section,Cancer Innovation Laboratory,Center for Cancer Research,National Cancer Institute,Frederick,MD,21702,USA

出　　处：《Bioactive Materials》2024年第11期640-656,共17页生物活性材料（英文）

基　　金：National Science Foundation Graduate Research Fellowship Program under Grant(award#2020300397);Any opinions,findings,conclusions,or recommendations expressed in this material are those of the author(s)and do not necessarily reflect the views of the National Science Foundation.

摘　　要：Decellularized extracellular matrices (dECM) have strong regenerative potential as tissue engineering scaffolds;however, current clinical options for dECM scaffolds are limited to freeze-drying its native form into sheets. Electrospinning is a versatile scaffold fabrication technique that allows control of macro- and microarchitecture. It remains challenging to electrospin dECM, which has led researchers to either blend it with synthetic materials or use enzymatic digestion to fully solubilize the dECM. Both strategies reduce the innate bioactivity of dECM and limit its regenerative potential. Herein, we developed a new suspension electrospinning method to fabricate a pure dECM fibrous mesh that retains its innate bioactivity. Systematic investigation of suspension parameters was used to identify critical rheological properties required to instill “spinnability,” including homogenization, concentration, and particle size. Homogenization enhanced particle interaction to impart the requisite elastic behavior to withstand electrostatic drawing without breaking. A direct correlation between concentration and viscosity was observed that altered fiber morphology;whereas, particle size had minimal impact on suspension properties and fiber morphology. The versatility of this new method was demonstrated by electrospinning dECM with three common decellularization techniques (Abraham, Badylak, Luo) and tissue sources (intestinal submucosa, heart, skin). Bioactivity retention after electrospinning was confirmed using cell proliferation, angiogenesis, and macrophage polarization assays. Collectively, these findings provide a framework for researchers to electrospin dECM for diverse tissue engineering applications.

关 键 词：ELECTROSPINNING EXTRACELLULAR matrix BIOLOGICAL scaffolds 

分 类 号：R318[医药卫生—生物医学工程]