C-myc modulates the replication of RGNNV via glutamine-mediated ATP production in grouper fin cells  

作　　者：Minshan Yao Hong Chen Junjie Tao Lixiang Wei Ying Tang Junyan Lin Fei Shi Fanbin Zhan Yanan Li Jun Li Zhendong Qin Li Lin 

机构地区：[1]College of Animal Sciences and Technology,Zhongkai University of Agriculture and Engineering,Guangzhou,Guangdong,510225,China [2]School of Sciences and Medicine,Lake Superior State University,Sault Ste.Marie,MI,49783,USA

出　　处：《Aquaculture and Fisheries》2024年第6期929-936,共8页渔业学报（英文）

基　　金：jointly supported by the National Natural Science Foundation of China(31872606,31902409,31572657,U1701233).

摘　　要：C-myc is a proto-oncogene that plays an important role in a variety of diseases.There were a lot of research on the correlation between C-myc and human viruses.However,the study about C-myc related to aquatic species virus is very limited.In the present study,the qRT-PCR,cellular immunofluorescence and western blotting determination data reported that C-myc and glutaminase(GLS)genes were significantly upregulated when grouper fin cells(GF-1)were infected with red grouper nervous necrosis virus(RGNNV).After knocking down the C-myc gene,the mRNA and protein levels of GLS,capsid protein(CP)and RNA polymerase(RdRp)of RGNNV were significantly reduced in RGNNV-infected GF-1 cells and the overexpression of the C-myc gene remarkably promoted these genes,which indicated that the replication of the virus and GLS gene were positively regulated by C-myc in RGNNV-infected GF-1 cells.In addition,supplementation of exogenous ATP can partially restore viral replication when RGNNV-infected GF-1 cells were cultured in glutamine-free medium,which confirmed that the glutamine was decomposed into ATP to provide energy for viral replication.Further studies confirmed that overexpression of C-myc can increase the content of ATP in normal cells.To sum up,these data suggested that activation of C-myc gene affected viral replication by regulating GLS expression to drive glutamine dissolution.

关 键 词：Red grouper nervous necrosis virus(RGNNV) C-MYC GLUTAMINE ATP 

分 类 号：S96[农业科学—水产养殖]