Krill oil ameliorates benign prostatic hyperplasia by regulating G1-phase cell cycle arrest and altering signaling pathways and benign prostatic hyperplasia-associated markers  

作　　者：Hoon Kim Jongyeob Kim Byungdoo Hwang Sang Yong Park Ji-Yeon Shin Eun Byeol Go Jae Sil Kim Youngjin Roh Soon Chul Myung Seok-Joong Yun Yung Hyun Choi Wun-Jae Kim Sung-Kwon Moon 

机构地区：[1]Department of Food and Nutrition,Chung-Ang University,Anseong 17546,Republic of Korea [2]SD Biotechnologies,Seoul 07793,Republic of Korea [3]Department of Urology,College of Medicine,Chung-Ang University,Seoul 06974,Republic of Korea [4]Department of Urology,Chungbuk National University,Chungbuk 361-763,Republic of Korea [5]Department of Biochemistry,College of Oriental Medicine,Dongeui University,Busan 47340,Republic of Korea [6]Institute of Urotech,Cheongju,Chungcheongbuk-do 361-763,Republic of Korea

出　　处：《Food Science and Human Wellness》2024年第6期3311-3324,共14页食品科学与人类健康（英文）

基　　金：supported by the Basic Science Research Program through the National Research Foundation of Korea(NRF)funded by the Ministry of Education(2018R1A6A1A03025159).

摘　　要：Krill oil(KO)exhibits various biological activities,such as anti-inflammatory and antitumor effects.However,the inhibitory effects of benign prostatic hyperplasia(BPH)in vitro and in vivo have not yet been studied.This study investigated the anti-BPH effects of KO extracted by an enzymatic hydrolysis method.KO treatment inhibited the proliferation of WMPY-1 and BPH-1 cells by induction of G0/G1 phase arrest through the modulation of positive and negative regulators in both prostate cell types.KO treatment stimulated phosphorylation of c-Jun N-terminal kinase(JNK)and p38 signaling.In addition,KO changed the expression of BPH-related markers(5α-reductase,androgen receptor,FGF,Bcl-2,and Bax)and the activity of the proliferation-mediated NF-κB binding motif.KO-induced levels of proliferation-mediated molecules of prostate cells were attenuated in the presence of siRNA-specific p-38(si-p38)and JNK(si-JNK).Furthermore,the administration of KO alleviated prostate size and weight and the cell layer thickness of prostate glands in a testosterone enanthate-induced BPH rat model.KO treatment altered the level of dihydrotestosterone in serum and the expression levels of BPH-related markers in prostate tissues.Finally,KO-mediated inhibition of prostatic growth was validated by histological analysis.These results suggest that KO has an inhibitory effect on BPH in prostate cells in vitro and in vivo.Thus,KO might be a potential prophylactic or therapeutic agent for patients with BPH.

关 键 词：Proliferation G0/G1-phase cell cycle NF-κB DIHYDROTESTOSTERONE 

分 类 号：TS225.24[轻工技术与工程—粮食、油脂及植物蛋白工程] R697.3[轻工技术与工程—食品科学与工程]