Dendrobium offi cinale flowers flavonoids enriched extract protects against acute ethanol-induced gastric ulcers via AMPK/PI3K signaling pathways  

作　　者：Zhiyu Zhang Hualing Xie Mohamed A.Farag Zhenhao Li Qingping Wu Ping Shao 

机构地区：[1]Department of Food Science and Technology,Zhejiang University of Technology,Hangzhou 310014,China [2]Pharmacognosy Department,College of Pharmacy,Cairo University,Cairo 12613,Egypt. [3]Zhejiang ShouXianGu Botanical Drug Institute Co.,Ltd.,Hangzhou 321200,China [4]Guangdong Provincial Key Laboratory of Microbial Safety and Health,State Key Laboratory of Applied Microbiology Southern China,Institute of Microbiology,Guangdong Academy of Sciences,Guangzhou 510070,China [5]Eco-Industrial Innovation Institute Zhejiang University of Technology,Quzhou 324000,China

出　　处：《Food Science and Human Wellness》2024年第6期3661-3679,共19页食品科学与人类健康（英文）

基　　金：supported by National Key Research and Development Program for Intergovernmental International Science and Technology Innovation Cooperation(2024YFE0109500);National Natural Science Foundation of China(32072149).

摘　　要：Gastric ulcer is a widespread disease caused by various etiologies.Dendrobium offi cinale flowers exert several health benefits owing to their rich flavonoid content.In this study,protective effects and possible action mechanisms of D.offi cinale flowers’flavonoid enriched extract(DOFF)were assessed against gastric ulcer.The result of sodium nitrite-aluminum nitrate colorimetry showed that 52.34%of the total extractive was flavonoid,and ultra-high performance liquid chromatography time of flight mass spectrometer(UPLC-QTOF/MS)revealed the presence of 28 components in DOFF of which 14 belonged to flavonoids.In addition,in vivo assay revealed DOFF potential in reducing the formation of ethanol-induced gastric mucosal lesions,with drop-in ulcer index from 64.33±8.76 to 32.00±4.47.Similar results were revealed in human gastric mucosal epithelia cells,with cells viability to increase from 27.2%to 61.6%post DOFF administration.To analyzed the protect effect of DOFF,we used Western blotting and immunofluorometric assay to revealed the expression levels of key proteins in cell pathways.The results showed that DOFF(320μg/mL)could increase the level of oxidation marker protein(HO-1),apoptosis regulatory protein(Bcl-2)and autophagy marker(LC3β)by 50.84%,43.85%,and 59.21%compared with ethanol-treated group respectively.Further analyzed of the mitochondrial activity and apoptosis pathway,we found that DOFF appeared to mitigate against ethanolinduced gastric mucosal injury via AMPK/mTOR/ULK1 and PI3K/AKT autophagy signaling pathways.

关 键 词：Dendrobium offi cinale flower ETHANOL FLAVONOIDS Gastric injury Autophagy 

分 类 号：TS201.4[轻工技术与工程—食品科学] R285[轻工技术与工程—食品科学与工程]