Suppression of Cs FAD3 in a JA-dependent manner,but not through the SA pathway,impairs drought stress tolerance in tea  

作　　者：Na Chang Xiaotian Pi Ziwen Zhou Yeyun Li Xianchen Zhang 

机构地区：[1]State Key Laboratory of Tea Plant Biology and Utilization,Anhui Agricultural University,Hefei 230036,China

出　　处：《Journal of Integrative Agriculture》2024年第11期3737-3750,共14页农业科学学报（英文版）

基　　金：supported by the Science Foundation for Anhui Province,China(2022AH050919);the Anhui Provincial Key Research and Development,China(2022l07020019);the Anhui Province Science and Technology Major Project,China(202203a06020014);the National Key Research and Development Program of China(2021YFD1601103);the Anhui University Collaborative Innovation Project,China(GXXT-2020-080)。

摘　　要：The growth and yield of tea plants are seriously limited by drought stress.Fatty acid desaturases(FADs)contribute to the mediation of membrane fluidity in response to different stresses,although the role ofω-3 FAD(Omega-3fatty acid desaturase)-mediated damage induced by drought stress in tea plants is poorly understood.In this study,drought stress significantly promoted the synthesis of C18:3(linolenic acid)and the expression level of CsFAD3.Yeast experiments further demonstrated that CsFAD3 can convert C18:2 to C18:3,and that the 35S:GFP-CsFAD3fusion protein was localized in the endoplasmic reticulum of Nicotiana benthamiana cells.CsFAD3-silenced tea leaves exhibited poor drought tolerance,with a lower F_(v)/F_(m)and a higher malondialdehyde(MDA)content than the control plants.However,transgenic 35S:CsFAD3 Arabidopsis plants showed the opposite phenotypes.In addition,the jasmonic acid(JA)content and the expression levels of CsLOX2,CsLOX4,CsAOS,CsAOC3 and CsOPR2 were significantly reduced in CsFAD3-silenced leaves under drought stress.However,no substantial difference in the salicylic acid(SA)content was detected under normal or drought conditions.An analysis of Atcoi1(JA receptor)or Atnpr1(SA receptor)mutant Arabidopsis plants in 35S:CsFAD3 backgrounds further revealed that knockout of Atcoi1impaired the drought-tolerant phenotypes of CsFAD3 overexpression lines.Therefore,this study demonstrated that CsFAD3 plays a crucial role in drought tolerance by mediating JA pathways.

关 键 词：tea plants CsFAD3 drought stress jasmonic acid 

分 类 号：S571.1[农业科学—茶叶生产加工]