Autophagy-related protein PlAtg3 participates in vegetative growth,sporangial cleavage,autophagy and pathogenicity of Peronophythora litchii  

作　　者：Chengdong Yang Manfei Luo Xue Zhang Linlin Ye Ge Yu Yi Lü Yi Chen Taixu Chen Xuejian Wang Wanzhen Feng Qinghe Chen 

机构地区：[1]School of Breeding and Multiplication(Sanya Institute of Breeding and Multiplication),Hainan University,Sanya 572025,China [2]Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests of Ministry of Education,School of Tropical Agriculture and Forestry,Hainan University,Haikou 570228,China [3]Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests,Fuzhou 350013,China

出　　处：《Journal of Integrative Agriculture》2024年第11期3788-3800,共13页农业科学学报（英文版）

基　　金：supported by the grants from the Hainan Provincial Natural Science Foundation,China(321QN190 and 321CXTD437);the National Natural Science Foundation of China(32202246 and 32160614);the Open Project Program of Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests,China(MIMCP-202102);the Scientific Research Foundation of Hainan University,China(KYQD(ZR)-21042 and KYQD(ZR)-20080)。

摘　　要：Litchi downy blight,caused by the plant pathogenic oomycete Peronophythora litchii,is one of the most devastating diseases on litchi and resulted in huge economic losses.Autophagy plays an essential role in the development and pathogenicity of the filamentous fungi.However,the function of autophagy in oomycetes remain elusive.Here,an autophagy-related protein Atg3 homolog PlAtg3 was identified and characterized in P.litchii.The absence of PlATG3 through the CRISPR/Cas9 gene replacement strategy compromised vegetative growth and sexual/asexual development.Cytological analyses revealed that the deletion of PlATG3 impaired autophagosome formation with monodansylcadaverine(MDC)staining and significantly disrupted zoospore release due to defects of sporangial cleavage with FM4-64 staining.Atg8 is considered to be an autophagy marker protein in various species.Western blot analysis indicated that PlAtg3 is involved in degradation of PlAtg8-PE.Interestingly,PlAtg3 was unable to interact with PlAtg8 in yeast two hybrid(Y2H)assays,possibly due to the absence of the Atg8 family interacting motif(AIM)in PlAtg3.Furthermore,pathogenicity assays revealed that the deletion of PlATG3 considerably reduced the virulence of P.litchii.Taken together,our data reveal that PlAtg3 plays an important role in radial growth,asexual/sexual development,sporangial cleavage and zoospore release,autophagosome formation,and pathogenicity in P.litchii.This study contributes to a better understanding of the pathogenicity mechanisms of P.litchii and provides insights for the development of more effective strategies to control oomycete diseases.

关 键 词：PlAtg3 sporangial cleavage AUTOPHAGY PATHOGENICITY Peronophythora litchii 

分 类 号：S436.67[农业科学—农业昆虫与害虫防治]