脑神经环路常用技术及其在针灸领域应用研究  

Common Techniques for Studying Neural Circuits and Their Application in Acupuncture Research

作　　者：梁越 张宁[1] 赵耀 吕沛然 谢璐霜[2] 刘奇[1] 乔海法 余曙光[2] 赵娴[1] LIANG Yue;ZHANG Ning;ZHAO Yao;LYU Peiran;XIE Lushuang;LIU Qi;QIAO Haifa;YU Shuguang;ZHAO Xian(Shaanxi University of Chinese Medicine,Xianyang 712046,China;Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China)

机构地区：[1]陕西中医药大学,陕西咸阳712046 [2]成都中医药大学,四川成都610075

出　　处：《针灸临床杂志》2024年第11期6-12,共7页Journal of Clinical Acupuncture and Moxibustion

基　　金：国家自然科学基金,编号:82205297、82205284、82074567;陕西省自然科学基础研究计划,编号:2022JQ-942、2022JQ-890。

摘　　要：神经环路对大脑的信息传递与处理有着至关重要的作用。脑内神经环路异常是很多脑疾病临床表型的核心基础,探索神经环路对于预防与治愈脑疾病意义重大。如何能高效观测(即具有高时间、空间和细胞类型分辨率)神经环路是神经科学研究中一直探寻的方向。本研究就现阶段广泛应用的神经示踪技术、光遗传学、化学遗传学、钙成像-光纤记录及电生理技术等的应用研究进行探讨,对以上技术在针灸领域(吞咽障碍、消化和疼痛等)的应用进行述评,以期为神经环路相关研究提供新思路。Neural circuits play a crucial role in the transmission and processing of information in the brain.Abnormalities in the brain neural circuit are the core basis of the clinical phenotypes of neurological diseases,making the exploration of neural circuit significant for the prevention and treatment of these conditions.Achieving efficient observation of neural circuits,with high temporal,spatial and cell type resolution,has been a key objective in neuroscience research.This study aims to explore the current applications of widely used techniques such as neural tracing,optogenetics,chemogenetics,calcium imaging-fiber photometry and electrophysiology.Additional,it reviews the application progress of these technologies in the field of acupuncture,particularly in the treatment of conditions like dysphagia,digestion and pain.The goal is to provide new insights and references for neural circuit-related research.

关 键 词：神经环路 光遗传学 化学遗传学 钙成像-光纤记录技术 

分 类 号：R245-0[医药卫生—针灸推拿学]