A novel chorismate mutase effector secreted from root-knot nematode Meloidogyne enterolobii manipulates plant immunity to promote parasitism  

作　　者：Tuizi Feng Yuan Chen Zhourong Li Ji Pei Deliang Peng Huan Peng Haibo Long 

机构地区：[1]Key Laboratory of Integrated Pest Management on Tropical Crops,Ministry of Agriculture and Rural Affairs,Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China [2]Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests,Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China [3]State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China

出　　处：《Journal of Integrative Agriculture》2024年第12期4107-4119,共13页农业科学学报（英文版）

基　　金：supported by the Hainan Provincial Natural Science Foundation of China(323MS102 and 320QN307);Central Public-Interest Scientific Institution Basal Research Fund,China(1630042022008)。

摘　　要：Meloidogyne spp.is an economically important plant-parasitic nematode distributed worldwide.To fight with host immune system for successful parasitism,plant parasitic nematodes secrete effectors to promote infection.In this study,we identified one chorismate mutase(CM)effector from M.enterolobii,named Me-CM.Spatial and temporal expression assays exhibited Me-cm is expressed in esophageal glands and up-regulated at parasitic-stage juveniles.Me-CM affects the pathogenicity of M.enterolobii based on the reduced infection rate,number of galls,egg masses,eggs per mass and multiplication rate collected from RNA silencing experiments.We showed that Me-CM localized in the cytoplasm and nucleus of plant cells and decreased the expression level of the marker gene PR1 of salicylic acid(SA)pathway.Besides,constitutive expression of Me-cm in Arabidopsis thaliana significantly reduced salicylic acid concentration.These results suggested that M.enterolobii may secrete effector Me-CM to fight with plantimmunesystemsvia regulating SA signaling pathway when interacting with host plants,ultimately facilitating parasitism.

关 键 词：Meloidogyne enterolobii EFFECTOR chorismate mutase salicylic acid plant immunity 

分 类 号：S432.2[农业科学—植物病理学]