Vesicular transport-related genes in Diaphorina citri are involved in the process of Candidatus Liberibacter asiaticus infection  

作　　者：Yingzhe Yuan Tao Peng Aijun Huang Jun He Chenyang Yuan Tianyuan Liu Long Yi Xuejin Cui Xuefeng Wang Changyong Zhou 

机构地区：[1]National Citrus Engineering and Technology Research Center,Citrus Research Institute,Southwest University,Chongqing 400712,China [2]College of Life Sciences,Yulin College,Yulin 719000,China [3]National Navel Orange Engineering Research Center,Gannan Normal University,Ganzhou 341000,China [4]Key Laboratory of Entomology and Pest Control Engineering,College of Plant Protection,Southwest University,Chongqing 400715,China

出　　处：《Journal of Integrative Agriculture》2024年第12期4136-4146,共11页农业科学学报（英文版）

基　　金：supported by grants from the National Key Research and Development Program of China(2021YFD1400800);the National Natural Science Foundation of China(U23A0196 and 32160625);the Innovation Research 2035 Pilot Plan of Southwest University,China(SWU-5331000008);the Special Fund for Youth Team of Southwest University,China(SWUXJLJ202310);the Science and Technology Project of Jiangxi Province,China(20225BCJ22005)。

摘　　要：Asian citrus psyllid(ACP,Diaphorina citri)is the major vector of Candidatus Liberibacter asiaticus(CLas),which is a bacterial pathogen causing the devastating citrus Huanglongbing(HLB)disease.Diaphorina citri is known to carry CLas in a persistent and propagative manner.Some studies have suggested that CLas may use the vesicular structures of D.citri cells as its propagation organelles.However,the mechanisms by which CLas enters the D.citri cells and how vesicle-mediated trafficking is involved remain unclear.In this study,we monitored the titer change of CLas in D.citri nymphs during the process of CLas acquisition from feeding on infected citrus plants.We found that the titer of CLas increased with the acquisition access period.After infection,there was a significant upregulation in the expression of several vesicular transport-related genes in D.citri.The titer of CLas was significantly reduced in the midgut and whole insect body when endocytosis and the endosome network in D.citri were inhibited.Furthermore,silencing the D.citri clathrin-heavy chain gene also led to a reduction in the CLas titer in D.citri.These results suggest that CLas infection upregulates the genes related to vesicular transport in D.citri,which facilitates the invasion of endocytosis-dependent pathogens.

关 键 词：HUANGLONGBING ENDOCYTOSIS vesicular trafficking CLas acquisition 

分 类 号：S433[农业科学—农业昆虫与害虫防治]