Extrusion bioprinting of elastin-containing bioactive double-network tough hydrogels for complex elastic tissue regeneration  

作　　者：Di Wang Jinshi Zeng Hailin Zhu Siyu Liu Litao Jia Wenshuai Liu Qian Wang Senmao Wang Wei Liu Jiayu Zhou Huimin Chen Xia Liu Haiyue Jiang 

机构地区：[1]Plastic Surgery Hospital of Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing,P.R.China [2]SinoBioprint Biotech Ltd.,Shanghai,P.R.China

出　　处：《Aggregate》2024年第3期188-201,共14页聚集体（英文）

基　　金：Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences,Grant/Award Numbers:2021-I2M-1-052,2017-I2M-1-007;National Multidisciplinary Cooperative Diagnosis and Treatment Capacity Building Project for Major Diseases,Grant/Award Number:21025;National Natural Science Foundation of China,Grant/Award Numbers:82371796,81871575。

摘　　要：Despite recent advances in extrusion bioprinting of cell-laden hydrogels,using nat-urally derived bioinks to biofabricate complex elastic tissues with both satisfying biological functionalities and superior mechanical properties is hitherto an unmet challenge.Here,we address this challenge with precisely designed biological tough hydrogel bioinks featuring a double-network structure.The tough hydrogels con-sisted of energy-dissipative dynamically crosslinked glycosaminoglycan hyaluronic acid(o-nitrobenzyl-grafted hyaluronic acid)and elastin through Schiff’s base reac-tion,and free-radically polymerized gelatin methacryloyl.The incorporation of elastin further improved the elasticity,stretchability(∼170%strain),and tough-ness(∼45 kJ m-3)of the hydrogels due to the random coiling structure.We used this novel class of hydrogel bioinks to bioprint several complex elastic tissues with good shape retention.Furthermore,in vitro and in vivo experiments also demon-strated that the existence of elastin in the biocompatible bioinks facilitated improved cell behaviors and biological functions of bioprinted tissues,such as cell spreading and phenotype maintenance as well as tissue regeneration.The results confirmed the potential of the elastin-containing tough hydrogel bioinks for bioprinting of 3D complex elastic tissues with biological functionalities,which mayfind widespread applications in elastic tissue regeneration.

关 键 词：biological functionalities BIOPRINTING double-network ELASTIN hydrogel mechanical properties tough-ness 

分 类 号：O63[理学—高分子化学]