Payload-free protein nanoparticles target inflamed colons to restore intestinal barrier integrity for effectively treating inflammatory bowel diseases  

作　　者：Mei Yang Honglan Shen Suting Zhong Zongpu Xu Xiangyu Liu Weicheng Wu Chuanbin Mao Mingying Yang 

机构地区：[1]Institute of Applied Bioresource Research,College of Animal Science,Zhejiang University,Hangzhou,China [2]School of Materials Science and Engineering,Zhejiang University,Hangzhou,China [3]Food Science Institute,Zhejiang Academy of Agricultural Sciences,Hangzhou,China [4]Department of Biomedical Engineering,The Chinese University of Hong Kong,Hong Kong SAR,China

出　　处：《Aggregate》2024年第4期113-125,共13页聚集体（英文）

基　　金：Zhejiang Provincial Science and Technology Plan,Grant/Award Number:2021C02072-6;National Natural Science Foundation of China,Grant/Award Numbers:81911530223,32101095;Provincial Key Laboratory Construction Plans,Grant/Award Number:2020E10025;Plan of National and Zhejiang Provincial Youth Science and Technology Innovation Leader,Grant/Award Numbers:[2020]366,2018R52021;Zhejiang Province Public Welfare Technology Research Plans,Grant/Award Number:LGF21H060008;National Key Research and Development Program of China,Grant/Award Number:2023YFF1103900;China Agriculture Research System of MOF and MARA。

摘　　要：Anti-inflammatory compounds,delivered as a payload to the gastrointestinal tract(GIT)by carriers,still cannot treat inflammatory bowel diseases without avoid-ing side effects.Here,we developed payload-free protein nanoparticles(PNPs)that crossed GIT to retain in the colon and treat colitis by restoring intestinal bar-rier integrity by modulating gut microbiome and metabolome.Specifically,PNPs,orally administered to mice with acute colitis,reached the colon within three hours.Consequently,PNPs improve gut microbiota dysbiosis to reverse metabolism bal-ance,suppressing the expression of tumor-necrosis factorαand toll-like receptor 4 that restores the intestinal barrier integrity.PNPs then ameliorated colon inflam-mation and attenuated gut microbiota dysbiosis by exerting probiotic effects on gut microbiota,treating colitis in a week more effectively than the clinically often used 5-aminosalicylic acid without causing undesired side effects.Such PNPs repre-sent safe,sustainable,and cost-effective therapeutics for treating inflammatory and metabolic diseases by eliminating microbial and metabolomic imbalance.

关 键 词：gut metabolites gut microbiota inflammatory bowel diseases(IBDs) protein nanoparticles(PNPs) 

分 类 号：R574[医药卫生—消化系统]