Aggregation and aging of nanoparticle-protein complexes at interfaces studied by evanescent-light scattering microscopy  

作　　者：Wei Liu Yuwei Zhu Hang Jiang Lidan Zhou Yinan Li Jiahao Wu Jie Han Cheng Yang Jianzhong Jiang To Ngai 

机构地区：[1]The Key Laboratory of Synthetic and Biological Colloids,Ministry of Education&School of Chemical and Material Engineering,Jiangnan University,Wuxi,China [2]Department of Chemistry,The Chinese University of Hong Kong,Hong Kong,China [3]School of Science and Technology,Hong Kong Metropolitan University,Hong Kong,China

出　　处：《Aggregate》2024年第4期208-219,共12页聚集体（英文）

基　　金：National Natural Science Foundation of China,Grant/Award Number:22303033;Fundamental Research Funds for the Central Universities of China,Grant/Award Number:JUSRP123017;Wuxi“Taihu Light”Science and Technology Project-Basic Research,Grant/Award Number:K20231063;Hong Kong Metropolitan University,Grant/Award Number:RD/2023/2.1;Hong Kong Special Administration Region(HKSAR)General Research Fund,Grant/Award Numbers:CUHK14302120,2130704。

摘　　要：Plasma protein-induced aggregation of nanoparticles(NPs)is a crucial issue in many applications,such as drug delivery.Although great efforts have been made to inves-tigate the protein adsorption kinetics or protein-induced NPs coalescence in bulk solutions,limited evidence has been uncovered for interfacial circumstances.Diet,disease,medicine,or senility could thoroughly change interfacial physicochemical properties of the inner lining of blood vessels.Implants including stents and artificial heart valves also have varied and evolutionary interfaces.Hence,there is an urgent need to understand the mechanism behind the non-specific protein adsorption and NP-protein aggregation in such interfacial cases.Here,we use evanescent light scat-tering to observe polystyrene NPs‒fibrinogen aggregation at substrates with varying surface properties.A density-fluctuation correlation function is utilized to reveal the relaxation dynamics of the aggregates.Both time-resolved and spatial-correlated evi-dence shows that the aging process of such soft materials is out-of-equilibrium,where the dynamics faster and slower than exponential can coexist in one sin-gle relaxation process.Besides,corona formation,inner stress,and interconnection together determine the microstructure,local adhesion,and structural relaxation of the aggregates,which can further correspond to the protein-to-NP ratio as well as the surface chemistry of NPs and substrates.

关 键 词：AGGREGATE AGING evanescent light scattering nanoparticles protein corona 

分 类 号：O64[理学—物理化学]