HOXA9和LRP6在甲状腺癌中的表达及其在预后评估中的价值  

Expression of HOXA9 and LRP6 in thyroid carcinoma and their value in prognosis evaluation

作　　者：董超鹏 徐婷婷 周群 董唯一 徐梦丹 朱培 DONG Chaopeng;XU Tingting;ZHOU Qun;DONG Weiyi;XU Mengdan;ZHU Pei(Department of Breast and Thyroid Surgery,Heze Municipal Hospital in Shandong Province,Heze,Shandong 274000,China;Department of Anesthesiology,Heze Municipal Hospital in Shandong Province,Heze,Shandong 2740o0,China;Department of Pathology,Heze Municipal Hospital in Shandong Province,Heze,Shandong 274000,China)

机构地区：[1]山东省菏泽市立医院乳腺甲状腺外科,山东菏泽274000 [2]山东省菏泽市立医院麻醉科,山东菏泽274000 [3]山东省菏泽市立医院病理科,山东菏泽274000

出　　处：《中国实验诊断学》2024年第11期1306-1312,共7页Chinese Journal of Laboratory Diagnosis

摘　　要：目的探讨甲状腺癌组织中同源盒基因A9(HOXA9)、低密度脂蛋白受体相关蛋白6(LRP6)表达情况及其在预后评估中价值。方法收集山东省菏泽市立医院2018年1月至2020年1月150例甲状腺癌患者术中切除的癌组织(TC组),另以同期100例甲状腺瘤患者术中切除的组织为对照(腺瘤组)。免疫组化法检测组织HOXA9、LRP6蛋白表达,分析HOXA9、LRP6蛋白表达与临床病理特征关系。Kaplan-Meier法分析HOXA9、LRP6蛋白表达与生存率关系。甲状腺癌患者预后影响因素利用COX回归模型。结果TC组HOXA9阳性表达率(72.67%vs 16.00%)、LRP6阳性表达率(68.67%vs 14.00%)明显高于腺瘤组(P<0.05)。经卡方检验以及GEPIA数据库检索发现,甲状腺癌组织HOXA9与LRP6表达水平呈正相关(P<0.05)。HOXA9、LRP6阳性表达者临床分期为Ⅲ期、有淋巴结转移、细胞低分化者占比高于HOXA9、LRP6阴性表达(P<0.05)。HOXA9阳性表达总生存率(72.48%vs 92.68%)、LRP6阳性表达总生存率(71.84%vs 91.49%)低于HOXA9阴性表达、LRP6阴性表达(χ^(2)=6.829、6.508,P=0.009、0.011)。COX回归分析显示,临床分期Ⅲ期、HOXA9阳性表达、淋巴结转移、LRP6阳性表达是甲状腺癌患者不良预后的影响因素(P<0.05)。结论甲状腺癌组织中HOXA9、LRP6呈高表达,与临床病理特征及预后有关,可用于评估甲状腺癌预后指标。Objective To investigate the expression of homeobox gene A9(HOXA9) and low-density lipoprotein receptor-related protein 6(LRP6) in thyroid carcinoma and their value in prognosis evaluation.Methods Intraoperative resection of 150 patients with thyroid cancer(TC group) from Heze Municipal Hospital, Shandong Province from January 2018 to January 2020 were collected, in addition, 100 cases of thyroid tumor were taken as control group(adenoma group).Immunohistochemical method was applied to detect the expression of HOXA9 and LRP6 proteins, and the relationship between the expression of HOXA9 and LRP6 proteins and the clinicopathological characteristics was analyzed.Kaplan-Meier method was applied to analyze the relationship between HOXA9,LRP6 protein expression and survival rate.The prognostic factors of thyroid cancer patients were analyzed by COX regression model.Results The positive expression rates of HOXA9(72.67% vs 16.00%) and LRP6(68.67% vs 14.00%) in TC group were obviously higher than those in adenoma group(P<0.05).Chi-square test and GEPIA database search showed that there was a positive correlation between HOXA9 and LRP6 expression in thyroid cancer tissue(P<0.05).The proportions of clinical stage Ⅲ,lymph node metastasis and low differentiation in patients with HOXA9 and LRP6 positive expression were higher than those in patients with HOXA9 and LRP6 negative expression(P<0.05).The total survival rate of HOXA9 positive expression(72.48% vs 92.68%) and LRP6 positive expression(71.84% vs 91.49%) were lower than those of HOXA9 negative expression and LRP6 negative expression(χ^(2)=6.829,6.508,P=0.009,0.011).COX regression analysis showed that clinical stage Ⅲ,HOXA9 positive expression and LRP6 positive expression were the influencing factors of poor prognosis in patients with thyroid cancer(P<0.05).Conclusion The expression of HOXA9 and LRP6 in thyroid carcinoma is high, it is related to the clinicopathological characteristics and prognosis, and can be used to evaluate the prognosis of thyroid carcinoma.

关 键 词：甲状腺癌 同源盒基因A9 低密度脂蛋白受体相关蛋白6 预后 

分 类 号：R736.1[医药卫生—肿瘤]