三阴性乳腺癌患者紧密连接蛋白4、转录因子叉头框C1、表皮生长因子受体表达与化疗敏感性的关系分析  

Relationship between chemotherapy sensitivity and claudin-4,Foxc1,and EGFR expression in patients with triple-negative breast cancer

作　　者：张明慧 赵长燕 贺红梅 ZHANG Minghui;ZHAO Changyan;HE Hongmei(Department of Pathology,Qinhuangdao Maternal and Child Health Hospital,Qinhuangdao 066000,China)

机构地区：[1]河北省秦皇岛市妇幼保健院病理科,河北秦皇岛066000

出　　处：《转化医学杂志》2024年第4期572-575,共4页Translational Medicine Journal

基　　金：秦皇岛市科学技术研究与发展计划项目(202005A028)。

摘　　要：目的探讨三阴性乳腺癌患者紧密连接蛋白4(Claudin-4)、转录因子叉头框C1(Foxc1)、表皮生长因子受体(EGFR)表达及与化疗敏感性的关系。方法选取2019年5月至2021年5月河北省秦皇岛市妇幼保健院收治的123例三阴性乳腺癌患者作为研究对象,根据新辅助化疗治疗效果分为化疗敏感组(n=86)和不敏感组(n=37)。qRTPCR检测Claudin-4、Foxc1、EGFR的表达,分析三阴性乳腺癌患者化疗不敏感的因素,绘制受试者工作特征(ROC)曲线分析3项指标对三阴性乳腺癌化疗敏感性的预测价值。结果敏感组Claudin-4、Foxc1、EGFR mRNA低于不敏感组(P<0.05)。Logistic回归分析结果显示Claudin-4、Foxc1、EGFR高表达是影响三阴性乳腺癌化疗敏感性的危险因素(P<0.05)。ROC曲线显示,Claudin-4、Foxc1、EGFR联合检测的ROC曲线下面积(AUC)高于各指标单独检测(P<0.05)。结论三阴性乳腺癌化疗不敏感患者Claudin-4、Foxc1、EGFR高表达是三阴性乳腺癌化疗不敏感的危险因素,三者联合检测可有效预测三阴性乳腺癌化疗敏感性。Objective To investigate the expression of tight junction-associated protein 4(Claudin-4),forkhead box C1(FOXC1),and epidermal growth factor receptor(EGFR)in patients with triple-negative breast cancer and their correlation with chemotherapy sensitivity.Methods A total of 123 patients with triple-negative breast cancer admitted to Qinhuangdao Maternal and Child Health Hospital from May 2019 to May 2021 were included and divided into a chemotherapy-sensitive group(n=86)and an insensitive group(n=37)according to the therapeutic effect of neoadjuvant chemotherapy.The expressions of Claudin-4,FOXC1,and EGFR were detected by the qRT-PCR method.The factors contributing to chemotherapy insensitivity in patients with triple-negative breast cancer were analyzed,and the predictive value of these three markers for chemotherapy sensitivity was evaluated using an ROC curve.Results The mRNA levels of Claudin-4,FOXC1,and EGFR were significantly lower in the sensitive group compared to the insensitive group(P<0.05).Logistic regression analysis revealed that high expressions of Claudin-4,FOXC1,and EGFR were risk factors for chemotherapy insensitivity in triple-negative breast cancer patients(P<0.05).The ROC curve showed that the AUC of the combined detection of Claudin-4,FOXC1,and EGFR was higher than that of individual testing(P<0.05).Conclusion Claudin-4,FOXC1,and EGFR were highly expressed in patients with chemotherapy insensitivity to triple-negative breast cancer,which were identified as risk factors for chemotherapy insensitivity.Moreover,the combined detection of these three markers effectively predicted chemotherapy sensitivity in triple-negative breast cancer.

关 键 词：三阴性乳腺癌 壳牢素-4 基因 erbB-1 化疗敏感性 ROC曲线 曲线下面积 

分 类 号：R737.9[医药卫生—肿瘤]