纤维素酶辅助碱提汉麻籽分离蛋白工艺优化  

Optimization of Hemp Seed Protein Extraction by Cellulase Assisted Alkali Extraction

作　　者：刘轶波 刘成国[1] LIU Yibo;LIU Chengguo(College of Food Science and Technology,Hunan Agricultural University,Changsha 410128,China)

机构地区：[1]湖南农业大学食品科学技术学院,湖南长沙410128

出　　处：《现代食品》2024年第19期60-63,共4页Modern Food

摘　　要：汉麻籽分离蛋白(Hemp Seed Protein Isolate,HPI)是汉麻籽深加工过程中的产物之一,尚未被完全利用开发。本文采用酶法水解脱脂汉麻籽粉辅助碱提HPI,以提取率和沉淀率为指标,设计单因素实验和正交实验,研究碱提料液比、碱提温度、碱提pH值、酶解pH值对纤维素酶辅助碱提酸沉法提取汉麻籽蛋白法的影响,并评估对比产品质量。结果表明,最佳工艺条件为酶解pH=4.0,料液比1∶20,碱提温度45℃,碱提pH=10.0。此条件下制备HPI沉淀率达到了77.9%,蛋白含量为83.69%。Hemp seed protein isolate(HPI) is one of the products from the deep processing of hemp seeds,which has not been fully utilized and developed.In this paper,enzymatic hydrolysis of defatted hemp seed powder was adopted to assist the alkaline extraction of HPI.Single-factor and orthogonal experiments were designed with extraction yield and precipitation rate as indicators to investigate the effects of material-to-liquid ratio,extraction temperature,extraction pH value,and enzymatic hydrolysis pH value on the cellulase assisted alkaline extraction acid precipitation method for extracting hemp seed protein,and to evaluate and compare product quality.The results demonstrated that the optimal process conditions were an alkaline extraction and enzymatic hydrolysis pH value of 4.0,a material-to-liquid ratio of 1∶ 20,an alkaline extraction temperature of 45 ℃,and an alkaline extraction pH value of 10.0,the precipitation rate of HPI reached 77.9%,and the protein content was 83.69%.

关 键 词：汉麻籽蛋白 酶辅助提取 工艺优化 

分 类 号：TS201.1[轻工技术与工程—食品科学]