Liver tissue engineering using decellularized scaffolds:Current progress,challenges,and opportunities  

作　　者：Kamal H.Hussein Boyukkhanim Ahmadzada Julio Cisneros Correa Ahmer Sultan Silvana Wilken Bruce Amiot Scott L.Nyberg 

机构地区：[1]Department of Surgery,Mayo Clinic,Rochester,MN,United States [2]William J.von Liebig Center for Transplantation and Clinical Regeneration,Mayo Clinic,Rochester,MN,United States [3]Department of Surgery,Anesthesiology,and Radiology,College of Veterinary Medicine,Assiut University,Assiut,Egypt

出　　处：《Bioactive Materials》2024年第10期280-305,共26页生物活性材料（英文）

基　　金：support from the Fulbright Visiting Scholar Program(2023-2024).

摘　　要：Liver transplantation represents the only definitive treatment for patients with end-stage liver disease.However,the shortage of liver donors provokes a dramatic gap between available grafts and patients on the waiting list.Whole liver bioengineering,an emerging field of tissue engineering,holds great potential to overcome this gap.This approach involves two main steps;the first is liver decellularization and the second is recellularization.Liver decellularization aims to remove cellular and nuclear materials from the organ,leaving behind extracellular matrices containing different structural proteins and growth factors while retaining both the vascular and biliary networks.Recellularization involves repopulating the decellularized liver with appropriate cells,theoretically from the recipient patient,to reconstruct the parenchyma,vascular tree,and biliary network.The aim of this review is to identify the major advances in decellularization and recellularization strategies and investigate obstacles for the clinical application of bioengineered liver,including immunogenicity of the designed liver extracellular matrices,the need for standardization of scaffold fabrication techniques,selection of suitable cell sources for parenchymal repopulation,vascular,and biliary tree reconstruction.In vivo transplantation models are also summarized for evaluating the functionality of bioengineered livers.Finally,the regulatory measures and future directions for confirming the safety and efficacy of bioengineered liver are also discussed.Addressing these challenges in whole liver bioengineering may offer new solutions to meet the demand for liver transplantation and improve patient outcomes.

关 键 词：Bioengineered liver DECELLULARIZATION Recellularization REENDOTHELIALIZATION Biliary reconstruction 

分 类 号：R318[医药卫生—生物医学工程]