微塑料暴露对铜锈环棱螺抗氧化酶活性及肠道微生物组成的影响  

Impact of Microplastic Exposure on the Antioxidant Enzyme Activity and Intestinal Microbiota Composition of Bellamya aeruginosa

作　　者：张明 金熠杰 张驰 徐逸文 樊岑熠 潘雯静 李嘉腾 陈慧丽 金斌松 ZHANG Ming;JIN Yijie;ZHANG Chi;XU Yiwen;FAN Cenyi;PAN Wenjing;LI Jiateng;CHEN Huili;JIN Binsong(College of Life and Environmental Sciences,Hangzhou Normal University,Hangzhou 311121,China)

机构地区：[1]杭州师范大学生命与环境科学学院,浙江杭州311121

出　　处：《杭州师范大学学报（自然科学版）》2024年第6期565-571,共7页Journal of Hangzhou Normal University(Natural Science Edition)

基　　金：国家自然科学基金项目(31960254);国家重点研发计划项目(2018YFD0900906,2018YFD0900806);杭州师范大学本科生创新能力提升工程项目(CX2023142);杭州师范大学2023年星光计划项目.

摘　　要：为揭示微塑料的生态毒性效应,以铜锈环棱螺(Bellamya aeruginosa)为模式生物,选择2种不同粒径(100 nm和10μm)的微塑料进行为期12 d的暴露实验,监测铜锈环棱螺内脏团超氧化物歧化酶(SOD)的活性及还原型谷胱甘肽(GSH)与氧化型谷胱甘肽(GSSG)比值的变化,并分析微塑料暴露对其肠道微生物的影响.结果显示,相较于10μm微塑料,100 nm微塑料可显著诱导铜锈环棱螺的SOD活性,并持续降低其GSH/GSSG比值,造成更为严重的氧化损伤.同时,100 nm微塑料显著影响了螺肠道微生物群落组成,导致其α多样性增加、益生菌减少、致病菌增多.可见,微塑料特别是纳米塑料可以破坏铜锈环棱螺的抗氧化防线,改变其肠道微生物群落结构,从而产生潜在的生态毒性作用.In order to reveal the ecotoxic effects of microplastics,Bellamya aeruginosa was selected as the model organism,and two kinds of microplastics with different particle sizes(100 nm and 10μm)were selected for a 12-day exposure experiment.The activity of superoxide dismutase(SOD)and the reduced to oxidized glutathione ratio(GSH/GSSG)in the visceral mass were monitored,and the impacts of microplastic exposure on the intestinal microbiota were analyzed.The results showed that compared with 10μm microplastics,100 nm microplastics could significantly induce SOD activity and continuously reduce the GSH/GSSG ratio,resulting in more serious oxidative damage.At the same time,100 nm microplastics significantly affected the intestinal microbial community composition,resulting in an increase inαdiversity,a decrease in probiotics,and an increase in pathogenic bacteria.It can be seen that microplastics,especially nano-plastics,can destroy the antioxidant defense line and change the structure of intestinal microbial community in B.aeruginosa,thus producing potential ecotoxic effects.

关 键 词：微塑料 铜锈环棱螺 氧化应激 抗氧化酶 肠道微生物 

分 类 号：X171.5[环境科学与工程—环境科学]