本科生综合实验:磁性固相萃取-高效液相色谱测定茶叶样品中的苯脲类除草剂  

Comprehensive undergraduate experiment:determination of phenylurea herbicide in tea by magnetic solid-phase extraction-high performance liquid chromatography

作　　者：刘壮 苏瑛[1] 杨东伟[1] 李丽梅[1] 许旭[2] LIU Zhuang;SU Ying;YANG Dongwei;LI Limei;XU Xu(College of Science,Shenyang Agricultural University,Shenyang 110866,China;College of Chemistry,Liaoning University,Shenyang 110036,China)

机构地区：[1]沈阳农业大学理学院,辽宁沈阳110866 [2]辽宁大学化学院,辽宁沈阳110036

出　　处：《色谱》2024年第11期1094-1100,共7页Chinese Journal of Chromatography

基　　金：沈阳农业大学教育教学研究立项(2022-101);教育部产学合作协同育人项目(220604807094530);辽宁省普通高等教育本科教学改革研究项目(辽教办[2021]254号第30项);辽宁大学研究生优质在线课程建设与教学模式综合改革研究项目(YJG202201015);国家自然科学基金(32102086).

摘　　要：在教育部推进新农科建设和学科交叉融合的背景下,本研究设计了一项综合化学实验,旨在提升学生在生物质功能材料制备及农药残留检测中的实践技能。实验采用天然丝瓜络合成氮掺杂磁性多孔碳材料,应用于磁性固相萃取(MSPE)技术,结合高效液相色谱法(HPLC)检测茶叶样品中的苯脲类除草剂灭草隆。该实验流程包括材料合成、表征、MSPE条件优化、吸附性能研究及HPLC分析,体现了科学性和系统性,为学生提供了一个全面的学习平台。本实验不仅加深了学生对材料特性与应用之间关系的理解,而且提高了实验设计和问题解决能力。通过科学前沿、课程思政与实验教学的结合,不仅激发了学生的科研兴趣,培养了创新思维和实践能力,还加强了学生的社会责任感和历史使命感,实现了实验教学的全面育人目标,为培养具有全球视野和社会责任感的高素质人才奠定了基础。Against the backdrop of the Ministry of Education’s promotion of new agricultural science construction and interdisciplinary integration,a comprehensive chemistry experiment to enhance the practical skills of students in preparing biomass functional materials and detecting pesticide residues was designed.Natural loofah was utilized as a precursor in synthesizing nitrogen-doped magnetic porous carbon materials,which were then applied in a magnetic solid-phase extraction(MSPE)technique.Subsequently,high performance liquid chromatography(HPLC)was employed to analyze and detect the phenylurea herbicide monuron in tea.The experimental process included material synthesis,characterization,optimization of the MSPE conditions,adsorption performance studies,and HPLC,reflecting its scientific,systematic nature and providing a comprehensive learning platform for students.It not only deepens student understanding of the relationship between material characterization and application,but also improves their experimental design and problem-solving capacities.Moreover,by integrating cutting-edge science,ideological and political education,and experimental training,it not only stimulates student interest in scientific research and cultivates innovative thinking and practical skills,but also strengthens their feelings of social responsibility and historical mission.This approach realizes the comprehensive educational goals of experimental training and lays the foundation for nurturing high-quality talent with a global perspective and sense of social responsibility.

关 键 词：综合化学实验 磁性固相萃取 高效液相色谱 农药分析 生物质材料 

分 类 号：O658[理学—分析化学]