枣庄地区市售猪肉中非伤寒沙门菌分布及病原特征分析  

Distribution and pathogenic characteristics of non-typhoidal Salmonella in pork samples at retail in Zaozhuang area

作　　者：李会会[1] 张亮 李读兴 王莉莉 张涛[1] LI Huihui;ZHANG Liang;LI Duxing;WANG Lili;ZHANG Tao(Zaozhuang Center for Disease Control and Prevention,ShandongZaozhuang277000,China)

机构地区：[1]枣庄市疾病预防控制中心,山东枣庄277000

出　　处：《中国食品卫生杂志》2024年第8期928-933,共6页Chinese Journal of Food Hygiene

基　　金：2020年度枣庄市医药卫生科技发展计划项目(202044)。

摘　　要：目的 明确枣庄地区市售猪肉中非伤寒沙门菌(NTS)的血清型分布、药物敏感性情况和分子分型特征。方法 利用玻片凝集法对NTS分离株进行血清学分型,采用微量肉汤稀释法测定分离株对14种抗生素的耐药性,使用脉冲场凝胶电泳技术(PFGE)对分离株进行分子分型并采用BioNumerics 7.6软件聚类分析。结果 30株NTS分离株共分为15种血清型,主要为里森沙门菌26.67%(8/30)和德尔卑沙门菌16.67%(5/30)。NTS分离株对四环素耐药率为80.00%、氨苄西林76.67%、复方新诺明63.33%,多重耐药率为66.67%,有46.67%的菌株对氨苄西林、四环素、氯霉素和复方新诺明4种抗生素同时产生耐药。PFGE带型具有多态性,无明显的地域聚集性,里森沙门菌带型最为集中。结论 枣庄地区市售猪肉中的NTS分离株以里森沙门菌和德尔卑沙门菌为主,对四环素、氨苄西林和复方新诺明等抗生素耐药率高并且出现严重的多重耐药现象,分子分型图谱呈现多态性。Objective To analyze the serotype distribution,drug resistance and molecular typing of non-typhoidal Salmonella(NTS)strains in pork sold in Zaozhuang area.Methods NTS strains were serotyped by slide agglutination method.The broth microdilution method was performed to determine the resistance of NTS to 14 antibiotics.Pulsed field gel electrophoresis(PFGE)was applied for molecular typing,and the strain clustering was analyzed by BioNumerics 7.6 software.Results Thirty strains of NTS were divided into 15 serotypes,and the main serotypes were Salmonella rissen(26.67%,8/30)and Salmonella derby(16.67%,5/30).The resistance rate of NTS strains to tetracycline was 80.00%,ampicillin was 76.67%,compound sulfamethoxazole was 63.33%,and the multi-drug resistance rate was 66.67%.There were 46.67%of the strains were resistant to four antibiotics(ampicillin,tetracycline,chloramphenicol and compound sulfamethoxazole).The PFGE bands were polymorphic without obvious regional aggregation,and the most concentrated were those of S.rissen.Conclusion The NTS strains in the pork sold in Zaozhuang area are mainly S.rissen and S.derby.The drug resistance rates to tetracycline,ampicillin and compound sulfa are high with severe multi-drug resistance.The molecular typing map shows polymorphism.

关 键 词：非伤寒沙门菌 血清分型 药敏试验 分子分型 

分 类 号：R155[医药卫生—营养与食品卫生学]