神经病学领域循环游离DNA研究的分析前因素研究进展  

Pre-analytical factors for the study of circulating cell-free DNA in the field of neurology

作　　者：应超 赵立芳 蔡燕宁 Ying Chao;Zhao Lifang;Cai Yanning(Department of Neurobiology,Xuanwu Hospital,Capital Medical University,Beijing 100053,China;Beijing Research Center of Geriatric Disease,Beijing 100053,China;Key Laboratory of Neurodegenerative Disease,Ministry of Education,Beijing 100053,China;National Clinical Research Center for Geriatric Diseases,Beijing 100053,China;Central Laboratory,Xuanwu Hospital,Capital Medical University,Beijing 100053,China)

机构地区：[1]首都医科大学宣武医院神经生物学研究室,北京100053 [2]北京市老年病医疗研究中心,100053 [3]教育部神经变性病重点实验室,北京100053 [4]国家老年疾病临床医学研究中心,北京100053 [5]首都医科大学宣武医院中心实验室,北京100053

出　　处：《神经疾病与精神卫生》2024年第11期806-814,共9页Journal of Neuroscience and Mental Health

基　　金：科技创新2030-“脑科学与类脑研究”重大项目(2021ZD0201101);国家重点研发计划(2021YFC2501205)。

摘　　要：目前,以神经系统疾病为重点的临床专科对非侵入性、敏感度和特异度高的检测方法的需求尚未得到充分满足。循环游离DNA(cfDNA)是一种具有潜在临床价值的生物标志物,因其携带了丰富的诊断和预后信息,在神经病学领域得到了广泛应用。但由于各研究机构之间缺乏统一的、标准化的cfDNA分析操作程序,导致cfDNA在临床实践中的应用仍受到一定限制。本综述通过系统性讨论影响cfDNA质量和产量的分析前因素,旨在为cfDNA在神经病学的临床实践中的具体应用提供参考。Currently,the clinical specialties focused on neurological disorders have not fully met the demand for non-invasive detection methods with high sensitivity and specificity.Circulating cell-free DNA(cfDNA)is a biomarker with significant potential clinical value,as it carries extensive diagnostic and prognostic information,and has been widely applied in the field of neurology.However,the lack of standardized cfDNA analysis protocols among different research institutions has constrained its application in clinical practice.This review aims to systematically discuss the pre-analytical factors that influence cfDNA quality and yield,providing a reference for the specific application of cfDNA in clinical neurology.

关 键 词：神经病学 循环游离DNA 分析前因素 综述 

分 类 号：R741[医药卫生—神经病学与精神病学] R440[医药卫生—临床医学]