3D Printing of Tough Hydrogel Scaffolds with Functional Surface Structures for Tissue Regeneration  

作　　者：Ke Yao Gaoying Hong Ximin Yuan Weicheng Kong Pengcheng Xia Yuanrong Li Yuewei Chen Nian Liu Jing He Jue Shi Zihe Hu Yanyan Zhou Zhijian Xie Yong He 

机构地区：[1]State Key Laboratory of Fluid Power and Mechatronic Systems,School of Mechanical Engineering,Zhejiang University,Hangzhou 310027,People’s Republic of China [2]Liangzhu Laboratory,Zhejiang University,Hangzhou 310027,People’s Republic of China [3]Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province,College of Mechanical Engineering,Zhejiang University,Hangzhou 310027,People’s Republic of China [4]Stomatology Hospital,School of Stomatology,Zhejiang University School of Medicine,Hangzhou 310027,People’s Republic of China [5]State Key Laboratory of Advanced Welding and Joining,Harbin Institute of Technology,Harbin 150001,People’s Republic of China [6]Institute of Digital Medicine,Nanjing First Hospital,Nanjing Medical University,Nanjing 210006,People’s Republic of China

出　　处：《Nano-Micro Letters》2025年第2期18-45,共28页纳微快报（英文版）

基　　金：supported by the Innovative Research Group Project of the National Natural Science Foundation of China(T2121004);Key Programme(52235007);National Outstanding Youth Foundation of China(52325504).

摘　　要：Hydrogel scaffolds have numerous potential applications in the tissue engineering field.However,tough hydrogel scaffolds implanted in vivo are seldom reported because it is difficult to balance biocompatibility and high mechanical properties.Inspired by Chinese ramen,we propose a universal fabricating method(printing-P,training-T,cross-linking-C,PTC&PCT)for tough hydrogel scaffolds to fill this gap.First,3D printing fabricates a hydrogel scaffold with desired structures(P).Then,the scaffold could have extraordinarily high mechanical properties and functional surface structure by cycle mechanical training with salting-out assistance(T).Finally,the training results are fixed by photo-cross-linking processing(C).The tough gelatin hydrogel scaffolds exhibit excellent tensile strength of 6.66 MPa(622-fold untreated)and have excellent biocompatibility.Furthermore,this scaffold possesses functional surface structures from nanometer to micron to millimeter,which can efficiently induce directional cell growth.Interestingly,this strategy can produce bionic human tissue with mechanical properties of 10 kPa-10 MPa by changing the type of salt,and many hydrogels,such as gelatin and silk,could be improved with PTC or PCT strategies.Animal experiments show that this scaffold can effectively promote the new generation of muscle fibers,blood vessels,and nerves within 4 weeks,prompting the rapid regeneration of large-volume muscle loss injuries.

关 键 词：3D printing Tough hydrogel scaffold Functional surface structure Tissue regeneration BIOMATERIALS 

分 类 号：R318.08[医药卫生—生物医学工程] TQ427.26[医药卫生—基础医学] TP391.73[化学工程]