两株鹅源益生菌的分离鉴定及其对雏鹅尿酸代谢的影响  

Isolation and Characterization of Two Strains of Goose-Derived Probiotics and Their Effect on Goslings

作　　者：赵宇 王秋菊[1,2] 孟令滢 魏笑 于文龙 冷欣阳 ZHAO Yu;WANG Qiuju;MENG Lingying;WEI Xiao;YU Wenlong;LENG Xinyang(College of Animal Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319,China;Key Laboratory of Exploration and Innovative Utilization of White Goose Germplasm Resources in the Cold Region of Heilongjiang Province,Daqing 163319,China)

机构地区：[1]黑龙江八一农垦大学动物科技学院,大庆163319 [2]黑龙江省寒区白鹅种质资源发掘与创新利用重点实验室,大庆163319

出　　处：《动物营养学报》2024年第11期7409-7421,共13页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：黑龙江省“双一流”特色学科平台项目(HLJTSXK2022);黑龙江省科技成果转化与扶贫推广项目(HLJJYT201912);黑龙江省农业农村厅种业创新和发展项目([2024]07);黑龙江省畜牧技术推广专项(HLJNY202109)。

摘　　要：本试验旨在分离鉴定2株鹅源益生菌——副干酪乳杆菌和解淀粉芽孢杆菌,并研究其对雏鹅尿酸代谢的影响。本试验随机选取健康成年籽鹅30只,收集粪便,采用微生物培养法,分离、鉴定和筛选益生菌,将筛选到的副干酪乳杆菌WX2011-R和解淀粉芽孢杆菌WX2104-S扩大培养,用于后续试验。选取1日龄籽鹅雏鹅48只,随机分为8组,每组3个重复,每个重复2只。将2种益生菌制备成冻干粉后按浓度兑入1 mL蒸馏水中,每日灌服,分组情况如下:对照组(NC组)、模型组(MC组)、副干酪乳杆菌/解淀粉芽孢杆菌低剂量组(RLD组/SLD组),冻干粉剂量为0.01 g/mL;副干酪乳杆菌/解淀粉芽孢杆菌中剂量组(RMD组/SMD组),冻干粉剂量为0.10 g/mL;副干酪乳杆菌/解淀粉芽孢杆菌高剂量组(RHD组/SHD组),冻干粉剂量为1.00 g/mL,对照组和模型组灌服1 mL蒸馏水。对照组饲喂基础饲粮,模型组及试验组饲喂高蛋白质饲粮,试验期为14 d。结果表明:1)灌服2种益生菌冻干粉均可对雏鹅的尿酸、肌酐、尿素氮含量和黄嘌呤氧化酶活性产生影响,与模型组相比,其中副干酪乳杆菌中剂量组4个指标均显著下降(P<0.05),与模型组相比,解淀粉芽孢杆菌高剂量组4个指标均显著下降(P<0.05)。2)在对尿酸代谢相关转运蛋白的表达检测中,与模型组相比,副干酪乳杆菌中剂量组的尿酸转运蛋白1(URAT1)和葡萄糖转运蛋白9(GLUT9)蛋白表达量显著下降(P<0.05),有机阴离子转运体1(OAT1)和ATP结合盒亚家族G成员2(ABCG2)蛋白表达量显著上升(P<0.05);与模型组相比,解淀粉芽孢杆菌高剂量组GLUT9蛋白表达量显著下降(P<0.05),OAT1和ABCG2蛋白表达量显著上升(P<0.05)。3)在实时荧光定量PCR试验中,与模型组相比,副干酪乳杆菌中剂量组URAT1和GLUT9基因相对表达量显著下降(P<0.05),OAT1和ABCG2基因相对表达量显著上升(P<0.05);与模型组相比,解淀粉芽孢杆菌高剂量组GLUT9基因相对表达量�The purpose of this study was to isolate and identify two goose probiotics,Lacticaseibacillus paracasei and Bacillus amyloliquefaciens,and to study their effects on uric acid metabolism in goslings.In this experiment,30 healthy adult seed geese were randomly selected,feces were collected,and probiotics were isolated,identified and screened by microbial culture method.The screened Lacticaseibacillus paracasei WX2011-R and Bacillus amyloliquefaciens WX2104-S were expanded and cultured for subsequent experiments.Forty-eight one-day-old goslings were randomly divided into 8 groups with 3 replicates per group and 2 goslings per replicate.Two kinds of probiotics were prepared into lyophilized powder,then mixed into 1 mL distilled water according to the concentration and administered daily,divided into the following groups:control group(NC group),model group(MC group),Lacticaseibacillus paracasei/Bacillus amyloliquefaciens low-dose group(RLD group/SLD group),the lyophilized powder dose was 0.01 g/mL;in the medium dose group(RMD group/SMD group),the freeze-dried powder dose was 0.10 g/mL;high dose group(RHD group/SHD group)was given 1.00 g/mL freeze-dried powder,and control group and model group were given 1 mL distilled water.Piglets in the control group were fed a basal diet,and those in the model group and experimental group were fed a high protein diet.The experiment lasted for 14 days.The results showed as follows:1)the contents of uric acid,creatinine,urea nitrogen and xanthine oxidase activity of goslings were all affected by administration of two kinds of lyophilized probiotics powder.Compared with the model group,the four indexes of Lacticaseibacillus paracasei medium dose group were significantly decreased(P<0.05),and the four indexes of Bacillus amyloliquefaciens high dose group were significantly decreased compared with the model group(P<0.05).2)In the detection of the expression of transporters related to uric acid metabolism,the expression levels of uric acid transporter 1(URAT1)and glucose transporter 9(GL

关 键 词：副干酪乳杆菌 解淀粉芽孢杆菌 雏鹅:高蛋白质饲粮 尿酸代谢 

分 类 号：S835[农业科学—畜牧学]