大气CO_(2)浓度和温度升高对玉米农田黑土碳库及细菌群落的影响  

Effects of elevated atmospheric CO_(2) concentration and warming on soil carbon pool and microbial community composition in a maize-grown Mollisol

作　　者：司马鑫琪 李彦生[1] 于镇华[1] 刘俊杰[1] 顾海东 刘居东[1] 王光华[1] 吴俊江[3] 苗淑杰[4] 乔云发[4] 金剑[1,3] SIMA Xinqi;LI Yansheng;YU Zhenhua;LIU Junjie;GU Haidong;LIU Judong;WANG Guanghua;WU Junjiang;MIAO Shujie;QIAO Yunfa;JIN Jian(State Key Laboratory of Black Soils Conservation and Utilization,Northeast Institute of Geography and Agroecology,Chinese Academy of Sciences,Harbin 150081,China;University of Chinese Academy of Sciences,Beijing 100049,China;Key Laboratory of Soybean Cultivation of Ministry of Agriculture,Soybean Research Institute,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China;School of Ecology and Applied Meteorology,Nanjing University of Information Science and Technology,Nanjing 210044,China)

机构地区：[1]中国科学院东北地理与农业生态研究所黑土保护与利用全国重点实验室,黑龙江哈尔滨150081 [2]中国科学院大学,北京100049 [3]黑龙江省农业科学院大豆研究所农业农村部大豆栽培重点实验室/黑龙江省大豆栽培重点实验室,黑龙江哈尔滨150086 [4]南京信息工程大学生态与应用气象学院,江苏南京210044

出　　处：《土壤与作物》2024年第4期428-438,共11页Soils and Crops

基　　金：中国科学院国际大科学计划培育专项(131323KYSB20210004);中国科学院先导项目(XDA28020201);黑龙江省自然科学重点项目(ZD2021D001).

摘　　要：为研究气候变化对东北农田黑土碳库和相关微生物组特征的影响,本文以东北春玉米连作的黑土为研究对象,利用原位开顶式气候室(OTC)模拟长期大气CO_(2)浓度和温度升高,利用土壤物理分级技术将土壤有机碳(SOC)分组为颗粒(POC)和矿质结合有机碳(MOC),并通过16S rRNA高通量测序分析土壤细菌微生物群落结构,探讨气候变化影响黑土碳库稳定性的微生物作用机制。结果表明,与CK相比,连续12年大气CO_(2)浓度和温度升高没有改变SOC总量,但影响其周转率。温度升高显著增加了细颗粒有机碳(fPOC)的含量,CO_(2)和温度同时升高降低了MOC含量。CO_(2)浓度升高并未影响fPOC和MOC含量。温度或CO_(2)浓度升高显著改变了细菌群落结构,温度升高增加苔藓杆菌属(Bryobacter)的相对丰度,CO_(2)浓度升高增加了革兰氏阴性菌硝化螺旋菌属(Nitrospirae)的丰度。长期大气CO_(2)浓度和温度同时升高增加硝化螺旋菌属(Nitrospirae)的丰度,该菌属丰度的增加可能会进一步影响SOC稳定性。In this study,we used the open top climate chamber(OTC)to simulate the long-term elevated atmospheric CO_(2) concentration and warming.The soil samples were fractionated into cPOC(coarse particulate organic C),fPOC(fine particulate organic C)and MOC(mineral-associated organic C).The change of soil microbial community diversity was analyzed by 16SrRNA highthroughput sequencing,and the mechanism of long-term elevated atmospheric CO_(2) concentration and warming on soil carbon conversion in dry farming soils was explained.The results showed that 12-yr consecutive climate change did not change the total soil organic carbon,but the turnover rate of soil organic carbon was significantly accelerated.Warming significantly increased fPOC,but decreased MOC.Long-term warming significantly increased the relative abundance of Bryobacter,and this genus was positively correlated with MOC.Long-term elevated atmospheric CO_(2) concentration did not significantly change the contents of fPOC and MOC.Elevated atmospheric CO_(2) concentration changed the soil microbial community,and significantly increased the abundance of Gram-negative bacteria Nitrospirae.Long-term elevated atmospheric CO_(2) concentration and warming increases the turnover rate of SOC,while changes in community structure or genus abundance would further affect SOC stability.

关 键 词：土壤有机碳 气候变化 土壤颗粒态有机碳 矿物结合态有机碳 微生物群落 

分 类 号：S155.27[农业科学—土壤学]