4-Aminoazobenzene:A novel negative ion matrix for enhanced MALDI tissue imaging of metabolites  

作　　者：Ran Wu Dongxu Jiang Hao Hu Chenyu Yang Liang Qin Lulu Chen Zehui Hu Hualei Xu Jinrong Li Haiqiang Liu Hua Guo Jinxiang Fu Qichen Hao Yijun Zhou Jinchao Feng Qiang Wang Xiaodong Wang 

机构地区：[1]Key Laboratory of Mass Spectrometry Imaging and Metabolomics(Minzu University of China),State Ethnic Affairs Commission,Beijing 100081,China [2]College of Life and Environmental Sciences,Centre for Imaging&Systems Biology,Minzu University of China,Beijing 100081,China [3]College of Biological Sciences and Biotechnology,Beijing Forestry University,Beijing 100083,China [4]Department of Orthopaedic Surgery,Sir Run Run Shaw Hospital,Zhejiang University School of Medicine,Hangzhou 310016,China [5]Key Laboratory of Musculoskeletal System Degeneration and Regeneration Translational Research of Zhejiang Province,Hangzhou 310016,China

出　　处：《Chinese Chemical Letters》2024年第11期273-277,共5页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.31770384 and 21605164);the Youth Academic Team Project of MUC(No.10301-02200301);the Huayi Technology Innovation Center for Research Resources(No.HTIC P01RR2017001A);the Key Laboratory Construction Funds of State Ethnic Affairs Commission of China(No.10301-02200303)。

摘　　要：Endogenous metabolites play key functions in many important physiological and biochemical processes.The comprehensive in situ detection and direct imaging of metabolites in bio-tissues by matrix-assisted laser desorption/ionization mass spectrometry imaging(MALDI-MSI)is very important for understanding complex and diverse biological processes and has become an essential aspect of spatial omics.In this work,4-aminoazobenzene(AAB)was successfully screened and optimized as a new negative ion(-)MALDI matrix to enhance the in situ detection and imaging of metabolites in tissues using MALDIMSI.Obviously,AAB exhibited superior properties in terms of ultraviolet absorption,background ion interference,matrix morphology,and metabolite ionization efficiency.AAB was used for in situ detection and imaging of metabolites in rat brain and germinating Chinese yew seed tissue sections,where 264and 339 metabolite ion signals were successfully detected and imaged using(-)MALDI-MS,respectively.In addition,high-resolution imaging of mouse eyeball section using MALDI-tims TOF MSI with spatial resolution of up to 10μm was successfully carried out,showing that AAB is an efficient(-)MALDI matrix for capturing high-resolution images of metabolites in biological tissue sections.

关 键 词：MALDI-MSI 4-Aminoazobenzene METABOLITES In situ detection Tissue imaging 

分 类 号：Q503[生物学—生物化学] O657.63[理学—分析化学]