肺腺癌组织中自噬相关蛋白表达与临床病理特征及预后关系  

The relationship between the expression of autophagy-related proteins in lung adenocarcinoma tissues and clinicopathological characteristics and prognosis

作　　者：黄璐 周国江[1] 李永来 Huang Lu;Zhou Guojiang;Li Yonglai(Department of Pathology,Ankang Central Hospital,Ankang 725000,China)

机构地区：[1]陕西省安康市中心医院病理科,安康725000

出　　处：《中华转移性肿瘤杂志》2024年第5期463-468,共6页Chinese Journal of Metastatic Cancer

摘　　要：目的检测肺腺癌组织中自噬相关蛋白ATG5、ATG9、BNIP3和BNIP3L的表达并分析其与临床病理特征及患者预后之间的相关性。方法回顾性选取2017年12月至2020年12月陕西省安康市中心医院诊治的150例肺腺癌患者术中切除的肿瘤组织及癌旁组织作为研究对象。收集并整理患者性别、吸烟史、年龄、病理分期、淋巴结转移以及分化程度等临床资料。采用免疫组织化学染色法检测自噬相关蛋白ATG5、ATG9、BNIP3和BNIP3L的表达水平。比较不同自噬相关蛋白表达情况与生存率的关系。Kaplan-Meier法生存分析,Cox模型多因素分析影响预后的因素。结果肺腺癌组织中ATG5、ATG9、BNIP3和BNIP3L蛋白表达水平显著高于癌旁组织(均P<0.05)。ATG5蛋白表达与患者病理分期、淋巴结转移以及分化程度显著相关(均P<0.05);ATG9蛋白表达与病理分期和淋巴结转移显著相关(均P<0.05);BNIP3蛋白表达与各项临床参数均无相关性(均P>0.05);BNIP3L蛋白表达与患者吸烟史、淋巴结转移、分化程度显著相关(均P<0.05)。自噬相关蛋白阳性表达者3年生存率均显著低于阴性表达者(均P<0.05)。多因素分析显示病理分期Ⅲ期,分化程度低,ATG5、ATG9、BNIP3和BNIP3L蛋白阳性表达均是预后不良的危险因素(均P<0.05)。结论ATG5、ATG9、BNIP3和BNIP3L蛋白在肺腺癌组织中高表达,并与肺腺癌患者预后不良密切相关。Objective To detect the expression of autophagy-related proteins ATG5,ATG9,BNIP3,and BNIP3L in lung adenocarcinoma tissues and analyze their correlation with the clinicopathological characteristics and prognosis of lung adenocarcinoma patients.Methods A retrospective selection of tumor tissues and adjacent non-tumor tissues resected during surgery was made from 150 lung adenocarcinoma patients treated in Ankang Central Hospital in Shaanxi Province from December 2017 to December 2020.Clinical data such as gender,smoking history,age,pathological staging,lymph node metastasis,and differentiation degree were collected and organized.The expression levels of autophagy-related proteins ATG5,ATG9,BNIP3,and BNIP3L were detected by immunohistochemical staining.To compare the expression of different autophagy-related proteins with survival rates.The survival analysis was performed with the Kaplan-Meier method.Multivariate analysis of Cox regression was used to determine the prognostic factors in lung adenocarcinoma patients.Results In lung adenocarcinoma tissues,the expression levels of ATG5,ATG9,BNIP3,and BNIP3L proteins were significantly higher than in adjacent non-tumor tissues(all P<0.05).The expression of ATG5 protein was significantly correlated with the patient′s pathological stage,lymph node metastasis,and degree of differentiation(all P<0.05).The expression of ATG9 protein was significantly correlated with pathological stage and lymph node metastasis(all P<0.05).BNIP3 protein expression was not correlated with any clinical parameters(all P>0.05);BNIP3L protein expression was significantly correlated with the patient′s smoking history,lymph node metastasis,and degree of differentiation(all P<0.05).Patients with positive expression of autophagy-related proteins had a significantly lower three-year survival rate than those with negative expression(all P<0.05).Multivariate analysis showed that pathological stage III,low degree of differentiation,and positive expression of ATG5,ATG9,BNIP3,and BNIP3L proteins were r

关 键 词：肺腺癌 自噬蛋白 临床病理特征 预后 

分 类 号：R734.2[医药卫生—肿瘤]