Aβ3-10KLH疫苗改善SAMP8小鼠认知功能和脑衰老  

Improved cognitive function and brain aging in SAMP8 mice after Aβ3-10KLH vaccine

作　　者：刘欣[1,2] 曹云鹏[1] 赵传胜[1] LIU Xin;CAO Yunpeng;ZHAO Chuansheng(Department of Neurology,The First Hospital of China Medical University,Shenyang 110001,China;Department of NeurologyⅡ,Affiliated Hospital of Beihua University,Jilin 132011,China)

机构地区：[1]中国医科大学附属第一医院神经内科,沈阳110001 [2]北华大学附属医院神经二科,吉林吉林132011

出　　处：《中国医科大学学报》2024年第11期979-983,共5页Journal of China Medical University

基　　金：辽宁省科学技术计划(2023JH2/101300043)。

摘　　要：目的探讨Aβ3-10KLH主动免疫对SAMP8小鼠模型脑衰老的影响。方法采用Aβ3-10KLH疫苗免疫SAMP8小鼠,并设立对照组。通过Morris水迷宫实验观察行为学变化,HE染色观察海马组织形态变化,采用SA-β-gal染色检测海马衰老细胞比例,生化试剂盒检测氧化应激损伤指标丙二醛(MDA)和超氧化物歧化酶(SOD)活性变化,评估海马氧化应激损伤水平。结果Aβ3-10KLH疫苗能减缓SAMP8小鼠海马组织细胞衰老,提高SAMP8小鼠记忆能力(P<0.05);改善海马组织形态;减少海马组织衰老细胞比例(P<0.05),降低MDA含量(P<0.05),提高SOD活性(P<0.05)。结论Aβ3-10KLH疫苗能显著改善SAMP8小鼠认知功能,减缓海马神经细胞衰老,并减少氧化应激损伤。Objective This study aimed to investigate the effects of Aβ3-10KLH active immunization on brain aging in the SAMP8 rapid aging mouse model using oxidative stress as the entry point.Methods SAMP8 mice were immunized with the Aβ3-10KLH vaccine,and a control group was established.Behavioral changes were observed using the Morris water maze test,and hippocampal morphological changes were examined using hematoxylin and eosin staining.The proportion of senescent cells in the hippocampus was detected using SA-β-gal staining.Biochemical kits were used to measure the changes in oxidative stress injury indicators malondialdehyde(MDA)and superoxide dismutase(SOD)activities to assess oxidative stress damage in the hippocampus.Results The Aβ3-10KLH vaccine attenuated cellular aging in the hippocampi of SAMP8 mice.It also improved memory ability(P<0.05),ameliorated hippocampal tissue morphology,decreased the proportion of senescent cells in the hippocampus(P<0.05),lowered MDA content(P<0.05),and upregulated SOD activity(P<0.05).Conclusion The Aβ3-10KLH vaccine significantly improves cognitive function in SAMP8 mice,slows down the aging of hippocampal neurons,and reduces oxidative stress damage.

关 键 词：阿尔茨海默病 脑衰老 氧化应激 

分 类 号：R749.1[医药卫生—神经病学与精神病学]