结直肠癌中TXNIP和KLF9的表达对患者临床特征及预后的影响  

Impacts of TXNIP and KLF9 expressions in colorectal cancer on the clinical characteristics and prognosis of patients

作　　者：杨婕琳[1] 王晓媛 武雪亮[2] YANG Jielin;WANG Xiaoyuan;WU Xueliang(Department of Gastroenterology,The First Affiliated Hospital of Hebei North University,Zhangjiakou 075000,China;Department of General Surgery,The First Affiliated Hospital of Hebei North University,Zhangjiakou 075000,China)

机构地区：[1]河北北方学院附属第一医院消化内科,河北张家口075000 [2]河北北方学院附属第一医院普外科,河北张家口075000

出　　处：《中国医科大学学报》2024年第11期1005-1011,共7页Journal of China Medical University

基　　金：河北省科技厅重点研发计划(22377784D);张家口市级科技计划(2021050D)。

摘　　要：目的探讨结直肠癌(CRC)组织中硫氧还蛋白相互作用蛋白(TXNIP)和Krüppel样转录因子9(KLF9)的表达对患者临床特征及预后的影响。方法选取2017年1月至2020年12月在我院初诊为CRC并手术治疗的患者90例,收集手术切除的癌组织及配对癌旁组织。采用免疫组织化学法检测组织中TXNIP、KLF9蛋白表达水平,用Spearman法分析CRC组织中TXNIP、KLF9表达的相关性,分析CRC组织TXNIP、KLF9蛋白表达与患者临床特征的关系,用Kaplan-Meier生存曲线分析CRC组织TXNIP、KLF9蛋白表达与总生存率的相关性,用Cox回归分析CRC患者预后影响因素。结果直肠腺癌(READ)TXNIP、KLF9 mRNA表达水平明显低于正常组织(P<0.05),且TXNIP、KLF9不同表达水平与READ患者总生存率无关(P>0.05)。CRC癌组织TXNIP阳性表达率(32.22%)、KLF9阳性表达率(37.78%)明显低于癌旁组织(73.33%、76.67%)(P<0.05)。Spearman分析发现,CRC组织中TXNIP与KLF9阳性表达率显著相关(r=0.519,P<0.05);Spearman分析结果显示,TXNIP与KLF9表达呈正相关(P<0.05)。临床分期为Ⅲ期、淋巴结发生转移的患者TXNIP、KLF9阳性表达率低于Ⅰ~Ⅱ期、淋巴结无转移者(P<0.05)。Kaplan-Meier分析显示,TXNIP阳性表达患者总生存率(89.66%)高于阴性表达患者(67.21%),KLF9阳性表达患者总生存率(88.24%)高于阴性表达患者(66.07%)(P<0.05)。多因素Cox回归发现,TNM分期为Ⅲ期(HR=2.752,95%CI:1.531~4.944)、TXNIP表达阴性(HR=2.036,95%CI:1.254~3.302)、KLF9表达阴性(HR=1.862,95%CI:1.236~2.804)是预后危险因素(P<0.05)。结论TXNIP、KLF9在CRC组织中低表达,且与TNM分期、淋巴结转移及较差的生存率有关。Objective To investigate TXNIP and KLF9 expressions in the tissues of colorectal cancer(CRC)and their impact on clinical characteristics and prognosis.Methods This study included 90 CRC patients who were admitted to our hospital between January 2017 and December 2020.The cancer tissue and paired adjacent tissues removed surgically were collected.We performed detection and analysis of TXNIP and KLF9 protein expression levels in cancer and adjacent tissues and their correlation with clinical characteristics and overall survival rate of patients.Results GEPIA database analysis showed that TXNIP and KLF9 mRNA expression levels in READ tissues were significantly lower than those in normal tissues(P<0.05).The positive expression rates of TXNIP and KLF9 in CRC tissues were 32.22%and 37.78%,respectively,which were lower than those in adjacent tissues(73.33%and 76.67%,respectively;P<0.05).TXNIP and KLF9 expressions were positively correlated(r=0.519,P<0.05),and GEPIA database retrieval showed a positive correlation between TXNIP and KLF9 expressions(P<0.05).TXNIP and KLF9 expressions in patients with clinical stageⅢand lymph node metastasis were lower than those in patients with stageⅠ/Ⅱand non-lymph node metastasis(P<0.05).The overall survival rate of patients with TXNIP and KLF9 expressions was higher than that of patients without TXNIP and KLF9 expressions.Negative expression of TXNIP and KLF9 in TNM stageⅢwas a prognostic risk factor(P<0.05).Conclusion TXNIP and KLF9 expression levels are low in CRC tissues and associated with TNM stage,lymph node metastasis,and poor survival.

关 键 词：结直肠癌 硫氧还蛋白相互作用蛋白 Krüppel样转录因子9 临床特征 预后 

分 类 号：R735.34[医药卫生—肿瘤]