β-烟酰胺单核苷酸的生物合成与纯化方法进展  

Advances in the biosynthesis and purification ofβ-nicotinamide mononucleotide

作　　者：李翔宇[1] 田大勇 林森[3] 姜兴益[1] 柴颖 罗彦波[1] 郭春生 郑超 庞永强[1] 侯宏卫[1,4] 李景虹 LI Xiangyu;TIAN Dayong;LIN Sen;JIANG Xingyi;CHAI Ying;LUO Yanbo;GUO Chunsheng;ZHENG Chao;PANG Yongqiang;HOU Hongwei;LI Jinghong(China National Tobacco Quality Supervision and Test Centre,Zhengzhou 450000,China;Inner Mongolia Kunming Cigarette Co.,Ltd.,Hohhot 010020,China;Department of Chemistry,Tsinghua University,Beijing 100080,China;Beijing Life Science Academy,Beijing 102200)

机构地区：[1]国家烟草质量监督检验中心,郑州450000 [2]内蒙古昆明卷烟有限责任公司,呼和浩特010020 [3]清华大学化学系,北京100080 [4]北京生命科技研究院,北京102200

出　　处：《生物医学工程研究》2024年第5期409-417,共9页Journal Of Biomedical Engineering Research

基　　金：中国烟草总公司重点研发项目(110202202027);中国烟草总公司郑州烟草研究院科研开发项目(2023410103600010)。

摘　　要：β-烟酰胺单核苷酸(nicotinamide mononucleotide,NMN)是哺乳动物体内烟酰胺腺嘌呤二核苷酸(nicotinamide adenine dinucleotide,NAD+)合成的关键前体物质之一,其具备抑制神经和血管老化、改善慢性疾病的作用,在保健食品和医药领域有着广阔的应用前景。微生物合成NMN主要通过代谢工程和合成生物学等方法改造获得,是一种经济、可持续的来源方式。但在微生物细胞中,NMN的代谢途径和调控机制尚不清晰,菌株生产效率低。因此,综述了NMN生物合成以及纯化工艺的相关进展,并介绍了未来发展趋势,可为相关研发和应用提供借鉴和参考依据。β-nicotinamide mononucleotide(NMN)is one of the key precursor substances for the synthesis of nicotinamide adenine dinucleotide(NAD+)in mammals.MNN can inhibit neural and vascular aging,improve the chronic diseases,and has broad application prospects in health food and medicine.Microbial synthesis of NMN is an economical and sustainable source,mainly obtained through rational methods such as metabolic engineering and synthetic biology.However,due to the long metabolic pathways,complex regulatory mechanisms,and unclear nature of NMN in microbial cells,production strains still face issues.This review summarizes the relevant progress in NMN biosynthesis and purification process applications,and briefly looks forward to future development trends,which can provide reference and guidance for subsequent related research and application.

关 键 词：β-烟酰胺单核苷酸 烟酰胺腺嘌呤二核苷酸 生物发酵 代谢工程 合成生物学 

分 类 号：R318[医药卫生—生物医学工程] Q81[医药卫生—基础医学]