广藿香水提物对溃疡性结肠炎小鼠肠屏障的影响  

Effect of Pogostemon cablin Aqueous Extract on Gut Barrier in Mouse Model of Ulcerative Colitis

作　　者：晋欣 张楠 梅晓亮 孙世豪 赵丽雯 严宝飞 JIN Xin;ZHANG Nan;MEI Xiaoliang;SUN Shihao;ZHAO Liwen;YAN Baofei(College of Pharmacy,Jiangsu Health Vocational College,Nanjing 211800,China;School of Chemistry and Molecular Engineering,Nanjing University of Technology,Nanjing 211800,China)

机构地区：[1]江苏卫生健康职业学院药学院,江苏南京211800 [2]南京工业大学化学与分子工程学院,江苏南京211800

出　　处：《食品工业科技》2024年第24期319-327,共9页Science and Technology of Food Industry

基　　金：江苏省大学生创新创业计划项目(202114255002Y);江苏卫生健康职业学院校级重点科研项目(JKB202004);江苏省高职院校青年教师企业实践培训资助项目(2024QYSJ009)。

摘　　要：目的:基于肠屏障研究广藿香水提物对溃疡性结肠炎(ulcerative colitis,UC)小鼠改善的作用及机制。方法:将小鼠随机分为空白组、模型组、美沙拉嗪组、广藿香水提物低、高剂量组;自由饮用2.5%葡聚糖硫酸钠(dextran sodium sulfate,DSS)10 d诱导小鼠UC模型,造模同时灌胃干预;检测血清白介素6(interleukin-6,IL-6)、白介素1β(IL-1β)和肿瘤坏死因子α(tumor necrosis factor α,TNF-α)水平;苏木素-伊红和高碘酸-席夫染色观察结肠病理变化;免疫组化法检测结肠紧密连接蛋白1(znula ocludens-1,ZO-1)、闭合连接蛋白1(occludin-1)和粘蛋白2(mucin 2,MUC2)蛋白表达;高通量测序技术分析结肠内容物肠道菌群变化。结果:DSS诱导的小鼠经广藿香水提物干预后,血清IL-6、IL-1β和TNF-α水平明显降低(P<0.05,P<0.01);结肠炎性浸润、上皮细胞结构破坏和杯状细胞数量减少等病理得到改善;结肠ZO-1、occludin-1和MUC2蛋白表达明显升高(P<0.05,P<0.01);肠道菌群α多样性sobs、Shannon和heip指数明显升高(P<0.05,P<0.01);肠道菌群β多样性主成分分析、主坐标分析和非度量多维尺度分析趋于健康小鼠;肠道菌群属水平优势物种隆布茨菌属(Romboutsia)、双歧杆菌属(Bifidobacterium)、布劳提菌属(Blautia)和unclassified_f__Lachnospiraceae丰度明显纠正(P<0.05,P<0.01)。结论:广藿香水提物对于DSS诱导的UC小鼠具有明确的改善作用,可能是其通过改善肠屏障而实现。Objective:To explore the ameliorative effects and mechanisms of Pogostemon cablin aqueous extract(PCAE)on mice with ulcerative colitis(UC)based on gut barrier research.Methods:Mice were randomly divided into five groups:Control,model,mesalazine,low-dose of PCAE and high-dose of PCAE groups.A UC model was induced in mice by freely drinking 2.5%dextran sodium sulfate(DSS)for 10 d,with simultaneous oral administration of interventions.The study measured levels of serum interleukin-6(IL-6),interleukin-1β(IL-1β),and tumor necrosis factorα(TNF-α).Pathological alterations in the colon were observed using hematoxylin-eosin and periodic acid-schiff staining.The expression of the colonic tight junction proteins zonula occludens-1(ZO-1),occludin-1,and mucin 2(MUC2)were assessed via immuno-histochemistry.High-throughput sequencing technology was utilized to analyze changes in the gut microbiota of colonic contents.Results:Following intervention with PCAE,DSS-induced mice exhibited a significant reduction in serum levels of IL-6,IL-1β,and TNF-α(P<0.05,P<0.01).Pathological improvements in colon inflammation,epithelial cell structural damage,and reduced goblet cell numbers were observed.Additionally,the expression of colonic ZO-1,occludin-1,and MUC2 proteins significantly increased(P<0.05,P<0.01).Furthermore,theα-diversity indices of the gut microbiota including sobs,Shannon,and heip exhibited a marked increase(P<0.05,P<0.01),while theβ-diversity analysis through principal component analysis,principal co-ordinates analysis,and non-metric multidimensional scaling analysis tended towards that of healthy mice.Notably,there was a significant correction in the abundance of dominant species at the genus level,including Romboutsia,Bifidobacterium,Blautia,and unclassified_f__Lachnospiraceae(P<0.05,P<0.01).Conclusion:PCAE exhibited a clear ameliorative effect on DSS-induced UC in mice,potentially achieved through the improvement of the gut barrier.

关 键 词：溃疡性结肠炎 广藿香水提物 肠屏障 肠上皮屏障 肠道菌群 

分 类 号：R9313[医药卫生—生药学]