基于转录组测序探究OSW-1抗肝癌的作用机制  

Evaluating the Anticancer Potential of Ornithogalum saundersiae Saponins on Hepatocellular Carcinoma:A Transcriptome Sequencing Method

作　　者：徐博 姜汀 金旺德 金星林[1] XU Bo;JIANG Ting;JIN Wangde;JIN Xinglin(The Affiliated Hospital of Yanbian University,Yanji 133000,China)

机构地区：[1]延边大学附属医院,吉林延吉133000

出　　处：《食品工业科技》2024年第24期346-352,共7页Science and Technology of Food Industry

基　　金：国家自然科学基金(8216140392)。

摘　　要：目的:基于转录组测序发掘虎眼万年青皂苷(Ornithogalum saundersiae Saponins,OSW-1)作用于肝癌细胞(HEP3B)后的关键通路及差异表达基因。方法:利用转录组测序技术检测OSW-1处理HEP3B的基因表达数据,通过测定的序列信息精确地分析转录本,筛选与细胞存活相关的表达差异基因,得到核心基因,并通过实验验证OSW-1是否通过调控核心靶点治疗肝细胞癌。结果:MTT结果表明,经OSW-1给药后HEP3B细胞活性明显降低,且呈浓度依赖性。通过转录组测序检测,共获得1381个存在明显表达差异的基因,并且多数基因富集在TNF信号通路。进一步通过免疫荧光及Western blot表明,OSW-1干预HEP3B细胞后,p-PI3K、p-AKT、p-NF-κB蛋白水平表达降低。结论:OSW-1可能通过调控炎症信号通路降低炎症反应,抑制HEP3B细胞活性,可作为潜在的治疗肝癌的药物,以及为保肝护肝的保健食品研发提供实验依据。To investigate the crucial pathways and differentially expressed genes following the action of Ornithogalum saundersiae Saponins(OSW-1)on liver cancer cells(HEP3B),this study utilized transcriptome sequencing technology to examine the gene expression data of HEP3B cells treated with OSW-1.By precisely analyzing the transcripts from the measured sequence information,differentially expressed genes related to cell survival were screened to identify core genes.Furthermore,experiments were conducted to verify whether OSW-1 could treat hepatocellular carcinoma by regulating the core targets.Results showed that the MTT assay revealed a significant reduction in HEP3B cell activity following OSW-1 treatment,which was dependent on the concentration.A total of 1381 genes showed significant differences in expression as a result of transcriptome sequencing,with a majority being involved in the TNF signaling pathway.Additionally,immunofluorescence and Western blot analysis indicated that the levels of p-PI3K,p-AKT,and p-NF-κB proteins were reduced in HEP3B cells after treatment with OSW-1.In conclusion,OSW-1 may suppress inflammation and reduce HEP3B cell activity by modulating inflammatory signaling pathways,suggesting its potential as a drug for liver cancer treatment and providing an experimental foundation for the development of healthy foods aimed at liver protection.

关 键 词：虎眼万年青皂苷 转录组测序 HEP3B细胞 差异表达基因 

分 类 号：R318.0[医药卫生—生物医学工程]