A new glucosyltransferase UGT78 from Iris sanguinea is a putative negative regulator in cadmium stress response  

作　　者：Gongfa Shi Guiling Liu Huijun Liu Lei Wang Aerdake Kuwantai Yu Du Ling Wang Xiaolei Xi Rusong Chai 

机构地区：[1]College of Landscape Architecture,Northeast Forestry University,Harbin 150040,People’s Republic of China [2]Taiyuan Botanical Garden,Taiyuan 030025,People’s Republic of China [3]Heilongjiang Forest Botanical Garden,Harbin 150040,People’s Republic of China

出　　处：《Journal of Forestry Research》2024年第6期344-355,共12页林业研究（英文版）

基　　金：supported by the Science and Technology Basic Resources Investigation Program of China(2019FY100500);the Fundamental Research Funds for the Central Universities(2572023CT18);the National Science Foundation(31670344).

摘　　要：Urbanization has resulted in a significant degra-dation of soil quality,subjecting plants to persistent abiotic stressors such as heavy metal pollution,salinization,and drought.UDP-glycosyltransferases(UGTs)participate in protein glycosylation,secondary metabolite synthesis,and detoxification of exogenous toxic substances.Iris sanguinea Donn ex Hornem exhibits a high degree of resistance to various abiotic stressors.To enhance the plant’s response to adversity,a novel glycosyltransferase belonging to the UGT78 family,encoding flavonoid 3-O-glucosyltransferase(UF3GT),was cloned from the monocot species I.san-guinea.Compared with the control group,overexpression of IsUGT78 enhanced sensitivity to cadmium stress,while showing no significant impact under NaCl and d-sorbitol treatments.Under cadmium treatment,arabidopsis exoge-nously transformed with the IsUGT78 gene possessed lower germination,fresh weight,root length,and chlorophyll con-tent and increased malondialdehyde content than the wild type arabidopsis.In addition,metabolomics in leaves led to the identification of 299 flavonoid metabolites,eight and 127 which were significantly up-and down-regulated,respec-tively,in the transgenic plants.Of note,all eight upregulated flavonoid compounds were glycosylated.Given that arabi-dopsis,which exogenously expresses the IsUGT78 gene,has reduced resistance to cadmium,IsUGT78 may lead to a reduced ability to cope with cadmium stress.

关 键 词：Iris sanguinea IsUGT78 Stress resistance CADMIUM GLUCOSYLTRANSFERASE 

分 类 号：X173[环境科学与工程—环境科学]