超高压提取法对五味子果实及藤茎中木脂素类成分含量的影响  

The Effect of Ultra-high Pressure Extraction Method on the Content of Lignans in the Fruit and Stemof Schisandra chinensis

作　　者：刘伟 张昊 李新殿[1] 李慧萍[1] 李伟[1] LIU Wei;ZHANG Hao;LI Xindian;LI Huiping;LI Wei(College of ChineseMedicinalMaterials,Jilin Agricultural University,Changchun 130118,China;College of Pharmacy,Beihua University,Jilin 132013,China)

机构地区：[1]吉林农业大学中药材学院,吉林长春130118 [2]北华大学药学院,吉林吉林132013

出　　处：《特产研究》2024年第6期87-95,共9页Special Wild Economic Animal and Plant Research

基　　金：吉林省科技发展计划项目(20200404027YY)。

摘　　要：本文主要探究了超高压提取法对五味子果实及藤茎中木脂素类成分含量的影响,为深入揭示五味子及其藤茎中有效成分提供理论基础。通过结合不同提取溶剂、不同部位以及不同压力情况下五味子有效成分的溶出,采用高效液相色谱法对五味子醇甲、五味子甲素和五味子乙素等7种木脂素类成分进行含量测定。结果表明,采用超声提取方式,木脂素含量达1.556%,有效成分溶出率为52.80%;经超高压提取后五味子果实中总木脂素含量可提高50.90%,有效成分溶出率提高13.49%,而藤茎中总木脂素提高14.55%,有效成分溶出率差异不大,当压力为400MPa,保压4~6 min含量最高。本研究表明,超高压提取法可以促进五味子果实和藤茎中木脂素类成分的溶出,其影响程度为五味子果实>五味子藤茎,为五味子有效成分的深度开发以及非药用部位综合开发利用奠定基础。In this study,the effect of ultra-high pressure extraction method on the content of lignans in the fruit and stemof Schisandra chinensis,which provided theoretical basis for further revealing the effective components in the fruit and stemof Schisandra chinensis.Combining the dissolution of the effective components of Schisandra chinensis under different extraction solvents,different parts and different pressure conditions,the content of seven lignans such as schisandrin A,schisandrin Aand schisandrin B were determined by high performance liquid chromatography.Using ultrasonic extraction method,the content of lignans reached 1.556%,and the dissolution rate of active ingredients was 52.80%.After ultra-high pressure extraction,the total lignan content in Schisandra fruits increased by 50.90%,and the dissolution rate of active ingredients increased by 13.49%.The total lignans increased by 14.55%,and the dissolution rate of the effective ingredients was not much different,the pressure was 400 MPa,and the holding pressure was the highest for 4~6 min.This study shows that the ultra-high pressure extraction method can promote the dissolution of lignans in the fruits and stem of Schisandra chinensis,the degree of influence:the fruit of Schisandra chinensis>the stems of Schisandra chinensis.It laid the foundation for further development of effective components and comprehensive development and utilization of non-medicinal parts of Schisandra chinensis.

关 键 词：超高压提取 五味子果实 藤茎 木脂素 含量测定 

分 类 号：TQ461[化学工程—制药化工]