基于重测序的中国李基因组InDel标记的开发及应用  

Development and Application of Prunus salicina InDel Markers Based on Genome Re-sequencing

作　　者：陈方策 胡平正 解为玮 王钲彭 钟创南 李海炎 何业华[1] 彭泽 万保雄 刘朝阳 CHEN Fangce;HU Pingzheng;XIE Weiwei;WANG Zhengpeng;ZHONG Chuangnan;LI Haiyan;HE Yehua;PENG Ze;WAN Baoxiong;LIU Chaoyang(Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in South China,Ministry of Agriculture and Rural Affairs,Colleage of Horticulture,South China Agricultural University,Guangzhou 510642,China;Chongqing Guopai Horticulture Co.,Ltd.,Chongqing 402560,China;Guangxi Academy of Special Crops,Guilin,Guangxi 541004,China)

机构地区：[1]农业农村部华南地区园艺作物生物学与种质创制重点实验室,华南农业大学园艺学院,广州510642 [2]重庆果派园艺有限公司,重庆402560 [3]广西特色作物研究院,广西桂林541004

出　　处：《园艺学报》2024年第11期2510-2522,共13页Acta Horticulturae Sinica

基　　金：广州市科技计划项目(202206010038);省级乡村振兴战略专项资金种业振兴项目(2022-NPY-00-029,2023-NBH-00-026)。

摘　　要：基于部分中国李种质资源的基因组重测序结果,挖掘出一系列候选InDel位点,并从中筛选出分布在8条染色体上且表现出稳定多态性的53个InDel标记。利用开发的53个InDel标记对79份李种质资源进行遗传多样性评价,主要等位基因频率平均为0.6593,平均基因多样性指数(H_(s))为0.4304,多态性信息含量(PIC)为0.3489,其中45个标记多态性信息含量较大(PIC>0.25),多态性较好。UPGMA聚类分析在遗传相似系数为0.623和0.666处,将79份材料划分为2个类群和10个亚群,分类结果与种质的地理分布有一定关联。利用其中的13个InDel标记将79份李种质进行区分,并构建了各种质13位的分子身份证。选择在亲本间具有多态性的InDel标记,对2个华南地区李品种正反杂交组合F_(1)代杂种的真伪进行了鉴定。Based on the genome re-sequencing results of several Chinese plum(Prunus salicina)germplasm resources,a series of candidate indel loci were mined,and 53 InDel markers which were distributed on different chromosomes and exhibited stable polymorphism,were screened out.The genetic diversity of 79 plum germplasm resources was evaluated by using 53 InDel markers.The average frequency of major alleles was 0.6593,the genetic diversity index(H_(s))was 0.4304,and the polymorphism information content(PIC)was 0.3489.Among them,45 markers had high polymorphism information content(PIC>0.25).Based on UPGMA cluster analysis,79 accessions were divided into 2 groups and 10 subgroups with genetic similarity coefficients of 0.623 and 0.666,respectively.The classification results were related to the geographical distribution of germplasm resources.The plum germplasms were distinguished by 13 InDel markers,and the germplasm specific molecular identity cards were constructed.By selecting InDel markers with polymorphism among parents,the F_(1)hybrid authenticity of positive and negative cross combinations of two plum cultivars in South China was identified.

关 键 词：李 INDEL标记 遗传多样性 分子身份证 F_(1)真实性鉴定 

分 类 号：S662.3[农业科学—果树学]