加味五子衍宗丸减轻雷公藤睾丸毒性作用机制的网络药理学研究  

Network pharmacological study on mechanism of modified Wuzi Yanzong pillin alleviating testicular toxicity of Tripterygium wilfordii

作　　者：孙利辉 韩姗姗[1,2] 代彦林 徐闪闪[1] 张建[1] 邢亚萍 丁樱[1,2] SUN Li-hui;HAN Shan-shan;DAI Yan-lin;XU Shan-shan;ZHANG Jian;XING Ya-ping;DING Ying(Pediatric Hospital,the First Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou 450000,China;Pediatric Medical College,Henan University of Chinese Medicine,Zhengzhou 450046,China)

机构地区：[1]河南中医药大学第一附属医院儿科医院,河南郑州450000 [2]河南中医药大学儿科医学院,河南郑州450046

出　　处：《中国药理学通报》2024年第12期2385-2391,共7页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金青年基金资助项目(No 82205187);中国民族医药学会科研项目(No 2021Z1182-400614);河南省中医药科学研究专项(No 2021ZY2031);河南中医药大学科研启动基金资助项目(No RSBSJJ2019-23)。

摘　　要：目的 运用网络药理学的方法,探讨加味五子衍宗丸减轻雷公藤睾丸毒性的作用机制。方法 运用多个数据库分析加味五子衍宗丸减轻雷公藤睾丸毒性的有效化学成分和作用靶点以了解作用机制。结果 加味五子衍宗丸的前5个化合成分分别是槲皮素、蔗糖、山奈酚、半乳糖、鞣花酸,前5个靶点分别是TP53、SRC、EP300、MAPK3、STAT3;KEGG通路富集分析主要富集在癌症通路、脂质与动脉粥样硬化、乙型肝炎、细胞凋亡、糖尿病并发症中的AGE-RAGE信号通路、PI3K-Akt信号通路等。结论 加味五子衍宗丸的活性成分主要通过干扰TP53、SRC、EP300、MAPK3、STAT3等靶点及调节细胞凋亡、PI3K-Akt信号通路、糖尿病并发症中的AGE-RAGE信号通路和脂质代谢等来减轻雷公藤睾丸毒性。Aim To explore the mechanism of modified Wuzi Yanzong pill to reduce testicular toxicity of Tripterygium wilfordii by means of network pharmacology.Methods The effective chemical composition and target of modified Wuzi Yanzong Pill to reduce testicular toxicity of Tripterygium wilfordii were analyzed by using multiple databases to understand the mechanism of action.Results The first five compounds of modified Wuzi Yanzong pill are quercetin,sucrose,kaempferol,galactose and ellagic acid,respectively.The first five targets were TP53,SRC,EP300,MAPK3,STAT3;KEGG pathway enrichment analysis mainly concentrated in cancer pathway,lipid and atherosclerosis,hepatitis B,apoptosis,diabetes complications AGE-RAGE signaling pathway,PI3K-Akt signaling pathway and other signaling pathways.Conclusions The active components of modified Wuzi Yanzong pill can reduce the testicular toxicity of Tripterygium wilfordii mainly by interfering with TP53,SRC,EP300,MAPK3,STAT3 and other targets,regulating apoptosis,PI3K-Akt signaling pathway,diabetes complications AGE-RAGE signaling pathway,lipid metabolism and other pathways.

关 键 词：雷公藤 睾丸毒性 加味五子衍宗丸 网络药理学 分子对接 作用机制 

分 类 号：R282.71[医药卫生—中药学] R289.5[医药卫生—中医学] R319R322.64