microRNA-143-3p在脑胶质瘤中的表达及其作用  

The expression and role of microRNA-143-3p in glioma

作　　者：安占森 郑翠红 刘京 贺泽丰 刘亮[3] 刘英姿[1] AN Zhan-sen;ZHENG Cui-hong;LIU Jing;HE Ze-feng;LIU Liang;LIU Ying-zi(Department of Neurosurgery,the Forth Hospital of Hebei Medical University,Shijiazhuang 050011,Hebei,China;不详)

机构地区：[1]河北医科大学第四医院神经外科,河北石家庄050011 [2]邢台市人民医院神经外科,河北邢台054001 [3]河北医科大学第四医院肿瘤研究所,河北石家庄050011

出　　处：《广东医学》2024年第11期1464-1472,共9页Guangdong Medical Journal

基　　金：河北省临床医学优秀人才培养项目(ZF2023064);河北省老年病防治项目(361006);河北省医学科学研究课题计划(20221318)。

摘　　要：目的通过检测miR-143-3p(microRNA,miR)在脑胶质瘤及正常脑组织中的表达差异,探讨其表达水平与脑胶质瘤的临床病理特征之间的相关性及其对脑胶质瘤生物学行为的潜在影响。方法收集2021年10月至2023年10月在河北医科大学第四医院神经外科手术切除的55例神经胶质瘤组织与15例来源于脑外伤及脑出血患者的正常脑组织。通过荧光定量逆转录聚合酶链反应检测miR-143-3p在胶质瘤组织与正常脑组织及人星形胶质细胞与胶质瘤细胞系(T98、U87、U251)中的表达水平。通过划痕实验、Transwell实验、细胞计数试剂盒8实验及流式细胞术检测过表达miR-143-3p后对胶质瘤细胞增殖、侵袭、迁移、细胞周期及细胞凋亡等功能的影响。结果在脑胶质瘤组织中,miR-143-3p的表达水平显著低于正常脑组织(P<0.0001)。miR-143-3p的低表达与胶质瘤的分化程度密切相关(P<0.001),而与年龄、性别、KPS评分等其他病理特征无显著关联(P>0.05)。miR-143-3p在T98、U87、U251胶质瘤细胞系中的表达显著低于人星形胶质细胞(P<0.01)。miR-143-3p的过表达显著抑制了肿瘤细胞的增殖、迁移和侵袭能力(P<0.01),肿瘤细胞周期在G_(0)/G_(1)期阻滞,其增殖指数显著下降、细胞凋亡率显著增加(P<0.01)。结论miR-143-3p在神经胶质瘤组织中低表达并与胶质瘤的恶性程度有一定关系,miR-143-3p可抑制胶质瘤细胞的增殖、侵袭和迁移并促进细胞凋亡。Objective To examine the differential expression of microRNA-143-3p(miR-143-3p)in gliomas and normal brain tissues,investigating its association with glioma clinicopathological features and its potential biological effects on glioma cell behavior.Methods Fifty-five glioma tissue samples and fifteen normal brain tissue samples from patients with traumatic brain injury or hemorrhage were collected from October 2021 to October 2023 at the Fourth Hospital of Hebei Medical University.Quantitative reverse transcription polymerase chain reaction(qRT-PCR)was used to determine miR-143-3p levels in glioma and normal brain tissues,as well as in human astrocytes and glioma cell lines(T98,U87,U251).The impact of miR-143-3p overexpression on glioma cell proliferation,invasion,migration,cell cycle progression,and apoptosis was evaluated using scratch assays,Transwell assays,CCK-8 assays,and flow cytometry.Results miR-143-3p expression was significantly lower in glioma tissues compared to normal brain tissues(P<0.0001).Low miR-143-3p expression correlated with glioma grade(P<0.001)but showed no significant association with age,gender,or KPS score(P>0.05).Similarly,miR-143-3p expression in glioma cell lines(T98,U87,U251)was significantly lower than in human astrocytes(P<0.01).Overexpression of miR-143-3p markedly inhibited glioma cell proliferation,migration,and invasion(P<0.01),induced cell cycle arrest in the G_(0)/G_(1) phase,decreased proliferation indices,and significantly increased apoptosis rates(P<0.01).Conclusion miR-143-3p is downregulated in glioma tissues,and its reduced expression is associated with glioma malignancy.miR-143-3p inhibits glioma cell proliferation,invasion,and migration while promoting apoptosis,suggesting its potential as a therapeutic target in glioma.

关 键 词：胶质瘤 微小RNA miR-143-3p 细胞增殖 

分 类 号：R739.41[医药卫生—肿瘤] R651.11[医药卫生—临床医学]