XRCC1蛋白、miR-7和c-erbB-2基因表达水平与非小细胞肺癌放疗抵抗的关系  

Relationship between XRCC1 protein,miR-7,c-erbB-2 gene expression levels and radiotherapy resistance in non-small cell lung cancer

作　　者：马婷婷 周立庆 苏欣宇 夏建洪 严研 MA Tingting;ZHOU Liqing;SU Xinyu;XIA Jianhong;YAN Yan(The Affiliated Huai an Hospital of Xuzhou Medical University,Huai'an,Jiangsu 223000,China)

机构地区：[1]徐州医科大学附属淮安医院,江苏淮安223000

出　　处：《中国临床研究》2024年第11期1725-1729,共5页Chinese Journal of Clinical Research

摘　　要：目的分析XRCC1蛋白、miR-7和c-erbB-2基因表达与非小细胞肺癌(NSCLC)放疗抵抗的关系及其预测价值。方法回顾性分析2020年1月至2023年6月徐州医科大学附属淮安医院就诊的初诊初治行放射治疗的ⅢB期及以上NSCLC患者100例。根据患者放疗效果,将其分为放疗敏感组(n=64)和放疗抵抗组(n=36),对比两组XRCC1蛋白、miR-7和c-erbB-2基因的表达水平。采用logistic回归分析NSCLC放疗抵抗的独立影响因素,采用受试者工作特征(ROC)曲线分析上述因素对NSCLC放疗抵抗的预测价值。结果放疗抵抗组患者肿瘤最大直径≥5 cm占比、XRCC1蛋白表达、miR-7和c-erbB-2基因表达量高于放疗敏感组(P<0.05)。肿瘤最大直径≥5 cm(OR=3.378,95%CI:1.438~7.931),XRCC1蛋白(OR=5.033,95%CI:1.994~12.708)、miR-7(OR=3.516,95%CI:1.994~6.198)和c-erbB-2基因(OR=4.036,95%CI:1.769~9.211)高表达是NSCLC放疗抵抗的独立危险因素(P<0.05)。c-erbB-2预测NCSLC放疗抵抗的AUC值最高,为0.963,其他依次为XRCC1蛋白(0.882)、miR-7(0.814)、肿瘤最大直径(0.648)。结论XRCC1蛋白、miR-7、c-erbB-2基因表达水平与NSCLC放疗抵抗有关,且对放疗抵抗具有良好预测价值。Objective To analyze the relationship between XRCC1 protein,miR-7,c-erbB-2 gene expression and radiotherapy resistance in non-small cell lung cancer(NSCLC)and their predictive value.Methods From January 2020 to June 2023,100 NSCLC patients with stageⅢB and above who were first diagnosed and treated with radiotherapy at The Affiliated Huai an Hospital of Xuzhou Medical University were retrospectively analyzed.The patients were divided into radiotherapy-sensitive group(n=64)and radiotherapy-resistant group(n=36)according to their radiotherapy effects,and the expression levels of XRCC1 protein,miR-7 and c-erbB-2 genes were compared between the two groups.Logistic regression was used to analyze the independent influencing factors of radiotherapy resistance in NSCLC,and the predictive value of the above factors on radiotherapy resistance in NSCLC was analyzed by ROC curve.Results The percentage of patients with tumor maximum diameter≥5 cm,XRCC1 protein expression,miR-7 and c-erbB-2 gene expression were higher in the radiotherapy-resistant group than in the radiotherapy-sensitive group(P<0.05).Tumors maximum diameter≥5 cm(OR=3.378,95%CI:1.438-7.931),XRCC1 protein high expressin(OR=5.033,95%CI:1.994-12.708),miR-7 gene high expression(OR=3.516,95%CI:1.994-6.198)and c-erbB-2 gene high expression(OR=4.036,95%CI:1.769-9.211)were independent risk factors for radiotherapy resistance in NSCLC(P<0.05).c-erbB-2 gene predicted NCSLC radiotherapy resistance with the highest AUC value of 0.963,and the others,in order of magnitude,were XRCC1 protein(0.882),miR-7(0.814),and tumor maximal diameter(0.648).Conclusion XRCC1 protein,miR-7,and c-erbB-2 gene expression levels were associated with NSCLC radiotherapy resistance and had good predictive value for radiotherapy resistance.

关 键 词：肺癌 非小细胞肺癌 放疗抵抗 放疗敏感 XRCC1蛋白 miR-7 C-ERBB-2基因 

分 类 号：R734.2[医药卫生—肿瘤]