单宁酸抑制膀胱癌细胞增殖及迁移侵袭作用的实验研究  

Experimental study on tannic acid inhibiting proliferation,migration and invasion of bladder cancer cells

作　　者：王昊雨 高文喜[2,3,4] 廖正明 郭凡 李焱[1] 周洁 郭朗 谢忱峰[1] 李国浩 高章远致 WANG Hao-yu;GAO Wen-xi;LIAO Zheng-ming(Hubei University of Chinese Medicine,Wuhan(430065),China)

机构地区：[1]湖北中医药大学,武汉430065 [2]湖北省中医院,武汉430061 [3]湖北省中医药研究院,武汉430061 [4]中医肝肾研究及应用湖北省重点实验室(湖北省中医院),武汉430061

出　　处：《中国中西医结合外科杂志》2024年第6期787-791,共5页Chinese Journal of Surgery of Integrated Traditional and Western Medicine

基　　金：武汉市科技局项目(2022020801020505)。

摘　　要：目的:探讨中药五倍子提取物单宁酸对膀胱癌EJ细胞和T24细胞的增殖、迁移和侵袭作用及其机制。方法:采用CCK-8、细胞生长计数实验方法检测不同浓度的单宁酸对膀胱癌EJ细胞和T24细胞增殖的抑制作用,应用平板克隆、细胞划痕和Transwell实验方法检测单宁酸对EJ和T24细胞增殖、迁移及侵袭能力的影响,Western Blot实验检测单宁酸对膀胱癌EJ和T24细胞凋亡、转移及维甲酸相关孤儿核受体C蛋白(RORC)的表达作用。结果:不同浓度的单宁酸对膀胱癌EJ细胞、T24细胞增殖的抑制作用呈剂量依赖性(P<0.05),EJ和T24细胞增殖、迁移、侵袭能力随着单宁酸浓度增加而降低(P<0.05);单宁酸上调膀胱癌EJ和T24细胞的凋亡相关蛋白PARP1、Caspase7以及E-cadherin的表达;同时下调转移相关蛋白N-cadherin、MMP-2、Vimentin、SNAI1的表达;EJ和T24细胞凋亡相关蛋白的表达与RORC的上调相关。结论:单宁酸通过上调RORC表达抑制膀胱癌EJ、T24细胞的增殖及迁移侵袭作用。Objective To investigate the effects of tannic acid from gallnut extract on proliferation,migration and invasion of EJ cells and T24 cells of bladder cancer and its mechanism.Methods CCK-8 and cell counting assay were used to detect the inhibitory effect of tannic acid at different concentrations on the proliferation of EJ and T24 cells.Plate cloning,cell scratch and Transwell assay were used to detect the effects of tannic acid on the proliferation,migration and invasion of EJ and T24 cells.The effects of tannic acid on apoptosis,metastasis and expression of retinoic acid-associated orphan nuclear receptor C protein(RORC)in bladder cancer EJ and T24 cells were detected by Western Blot assay.Results Tannic acid at different concentrations inhibited the proliferation of EJ and T24 cells in a dose-dependent manner(P<0.05),and the proliferation,migration and invasion of EJ and T24 cells decreased with the increase of tannic acid concentration(P<0.05).Tannic acid up-regulated the expression of apoptosis-related proteins PARP1,Caspase7 and E-cadherin in bladder cancer EJ and T24 cells.Meanwhile,the expression of transfer-related proteins N-cadherin,MMP-2,Vimentin and SNAI1 was down-regulated.The expression of EJ and T24 apoptosis-related proteins correlated with the up-regulation of RORC.Conclusion Tannic acid inhibited the proliferation and migration of bladder cancer cells EJ and T24 by up-regulating RORC expression.

关 键 词：单宁酸 膀胱癌 增殖 迁移与侵袭 维甲酸相关孤儿核受体C蛋白 

分 类 号：R737.14[医药卫生—肿瘤]