Rational design of water-soluble,homotypic keratins self-assembly with enhanced bioactivities  

作　　者：Jie Chen Yumei Wang Jia Deng Rui Qing Yaxi Yang Xiaoliang Chen Zibin Gao BochuWang Shilei Hao 

机构地区：[1]Key Laboratory of Biorheological Science and Technology,Ministry of Education,College of Bioengineering,Chongqing University,Chongqing,China [2]Department of Nuclear Medicine,Chongqing University Cancer Hospital,Chongqing,China [3]College of Environment and Resources,Chongqing Technology and Business University,Chongqing,China [4]State Key Laboratory of Microbial Metabolism,School of Life Sciences and Biotechnology,Shanghai Jiao Tong University,Shanghai,China [5]Department of Genetics,College of Biological Science,University of California—Davis,Davis,California,USA [6]State Key Laboratory Breeding Base Hebei Province Key Laboratory of Molecular Chemistry for Drugs,Hebei University of Science and Technology,Shijiazhuang,China

出　　处：《Aggregate》2024年第2期268-279,共12页聚集体（英文）

基　　金：National Natural Science Foundation of China,Grant/Award Numbers:11972099,82202340;Venture&Innovation Support Program for Chongqing Overseas Returnees,Grant/Award Number:cx2020079;Scientific and Fundamental Research Funds for the Central Universities,Grant/Award Numbers:2023CDJXY-050,2023CDJXY-051。

摘　　要：Recombinant keratins possess strong hemostatic and wound healing properties but suffer from poor water solubility that restricts their bioactivities in biomedical applications.Herein,we report the rational design and synthesis of water-soluble keratins using a simple methodology named the QTY code.In vitro biophysical analyses and molecular dynamic simulation demonstrated a 200-fold increase in the water solubility of QTY variant keratins without apparent structural changes compared to native proteins.Homotypic self-assembly was observed for the first time in recombinant keratins in an aqueous environment,without urea and after QTY modification.Cell and animal experiments showed the in situ gel-forming capability of QTY variant keratins with superior hemostatic and wound healing activities at the wound sites compared to native recombinant keratins.Our work not only presented a simple and feasible pathway to produce large amounts of water-soluble keratins using QTY modification but also validated the enhanced self-assembly,hemostasis,and wound healing properties of these novel keratin species that may open up new venues for biomedical applications.

关 键 词：KERATIN QTY code SELF-ASSEMBLY water solubility wound healing 

分 类 号：O62[理学—有机化学]